To assess longitudinal reproducibility of metrics of foveal density (peak cone density [PCD], cone density centroid [CDC], and 80th percentile centroid area) in participants with normal vision.
Participants ( n = 19; five male and 14 female) were imaged at two time points (average interval of 3.2 years) using an adaptive optics scanning light ophthalmoscope (AOSLO). Foveally centered regions of interest (ROIs) were extracted from AOSLO montages. Cone coordinate matrices were semiautomatically derived for each ROI, and cone mosaic metrics were calculated.
On average, there were no significant changes in cone mosaic metrics between visits. The average ± SD PCD was 187,000 ± 20,000 cones/mm 2 and 189,000 ± 21,700 cones/mm 2 for visits 1 and 2, respectively ( P = 0.52). The average ± SD density at the CDC was 183,000 ± 19,000 cones/mm 2 and 184,000 ± 20,800 cones/mm 2 for visits 1 and 2, respectively ( P = 0.78). The average ± SD 80th percentile isodensity contour area was 15,400 ± 1800 µm 2 and 15,600 ± 1910 µm 2 for visits 1 and 2, respectively ( P = 0.57).