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      Temporal dynamics of bacterial populations in recirculating aquaculture systems for sea urchin production Translated title: Dinámica temporal de poblaciones bacterianas en sistemas de recirculación para la producción acuícolas de erizo de mar

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          Abstract

          Abstract Introduction: Sea urchin aquaculture is a rising industry, and in consequence, there is a need to establish optimal culture parameters to ensure the health of the cultured animals. Objective: To evaluate the bacterial counts in the seawater of sea urchin (Arbacia dufresnii) aquaculture recirculating systems (RAS). Methods: The bacteriological water quality of two RAS containing sea urchins was determined. For approximately two months, weekly water samples were taken. The bacteriological quality was determined by counting total aerobic heterotrophic populations, lactic acid bacteria, enterobacterias and genus Vibrio. Physicochemical parameters were also measured. Results: There was no presence of disease or mortality. Enterobacteria and lactic acid bacteria were not detected from both RAS systems. The number of animals had an important effect on the observed difference in the count of total bacteria and Vibrio spp. In RAS 1 the maximum counts of total bacteria and Vibrio spp. were 2.8 x 105 ± 1.7 x 105 and 1.45 x 105 ± 3.6 x 104 UFC ml-1, respectively. In RAS 2 total bacteria and Vibrio spp. exhibited repetitive behavior over time influenced in part by water exchange and mainly by feeding. The results indicate that periodic water changes ensure a limited growth of bacterial strains as Vibrio and other bacteria. Conclusions: Our results suggests that the bacterial count levels recorded in this study can be used as a threshold or safety limit for Arbacia dufresnii aquaculture.

          Translated abstract

          Resumen Introducción: La acuicultura de erizos de mar es una industria en auge, y en consecuencia, existe la necesidad de establecer los parámetros de cultivo óptimos para garantizar la salud de los animales en cultivo. Objetivo: Evaluar los recuentos bacterianos en el agua de cultivo de los sistemas de recirculación acuícola (RAS) de erizo de mar Arbacia dufresnii. Métodos: Se determinó la calidad bacteriológica del agua de cultivo de dos RAS que contenían erizos de mar. Durante aproximadamente dos meses, se tomaron muestras de agua semanalmente. La calidad bacteriológica se determinó realizando recuento de las poblaciones heterótrofas aerobias totales, bacterias ácido lácticas, enterobacterias y bacterias del género Vibrio. También se midieron parámetros fisicoquímicos. Resultados: No se observaron patologías ni mortalidad. No se detectaron enterobacterias ni bacterias del ácido láctico en ninguno de los sistemas RAS. El número de animales cultivados tuvo un efecto importante en la diferencia observada en el recuento de bacterias totales y Vibrio spp. En el RAS 1 los recuentos máximos de bacterias totales y Vibrio spp. fueron 2.8 x 105 ± 1.7 x 105 y 1.45 x 105 ± 3.6 x 104 UFC ml-1, respectivamente. En RAS 2 los recuentos de bacterias totales y Vibrio spp. exhibieron un comportamiento repetitivo en el tiempo influenciado en parte por el recambio de agua y principalmente por la alimentación. Los resultados indican que los cambios periódicos de agua aseguran un crecimiento limitado de cepas bacterianas como Vibrio y otras bacterias. Conclusiones: Nuestros resultados sugieren que los niveles de recuento bacteriano registrados en este estudio pueden usarse como umbral o límite de seguridad para la acuicultura de Arbacia dufresnii.

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          R: a language and environment for statistical computing

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            Smoothing parameter selection in nonparametric regression using an improved Akaike information criterion

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              The Rising Tide of Antimicrobial Resistance in Aquaculture: Sources, Sinks and Solutions

              As the human population increases there is an increasing reliance on aquaculture to supply a safe, reliable, and economic supply of food. Although food production is essential for a healthy population, an increasing threat to global human health is antimicrobial resistance. Extensive antibiotic resistant strains are now being detected; the spread of these strains could greatly reduce medical treatment options available and increase deaths from previously curable infections. Antibiotic resistance is widespread due in part to clinical overuse and misuse; however, the natural processes of horizontal gene transfer and mutation events that allow genetic exchange within microbial populations have been ongoing since ancient times. By their nature, aquaculture systems contain high numbers of diverse bacteria, which exist in combination with the current and past use of antibiotics, probiotics, prebiotics, and other treatment regimens—singularly or in combination. These systems have been designated as “genetic hotspots” for gene transfer. As our reliance on aquaculture grows, it is essential that we identify the sources and sinks of antimicrobial resistance, and monitor and analyse the transfer of antimicrobial resistance between the microbial community, the environment, and the farmed product, in order to better understand the implications to human and environmental health.
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                Author and article information

                Journal
                rbt
                Revista de Biología Tropical
                Rev. biol. trop
                Universidad de Costa Rica (San José, San José, Costa Rica )
                0034-7744
                0034-7744
                March 2024
                : 72
                : suppl 1
                : e58882
                Affiliations
                [3] San Antonio Oeste orgnameCentro de Investigación aplicada y transferencia tecnológica en recursos Marinos “Almirante Storni” (CIMAS (CONICET, UnComa)) Argentina mcledon@ 123456gmail.com
                [2] Chubut orgnameUniversidad Nacional de la Patagonia San Juan Bosco orgdiv1Facultad de Ciencias Naturales y Ciencias de la Salud orgdiv2Laboratorio de Química de Organismos Marinos (LABQUIOM), Instituto Patagónico del Mar (IPAM) Argentina
                [1] Chubut orgnameCentro para el Estudio de Sistemas Marinos (CESIMAR-CCT CONICET-CENPAT) Argentina garces@ 123456cenpat-conicet.gob.ar
                Article
                S0034-77442024000200017 S0034-7744(24)07200000017
                10.15517/rev.biol.trop..v72is1.58882
                7b29c243-2c9d-44d5-adaf-24ad8d1a3047

                This work is licensed under a Creative Commons Attribution 3.0 International License.

                History
                : 02 November 2023
                : 02 July 2023
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 47, Pages: 0
                Product

                SciELO Costa Rica


                recirculating system,Arbacia dufresnii,culturable bacteria,bacteriological quality,physicochemical parameters,seawater quality.,bacterias cultivables,sistemas de recirculación,calidad bacteriológica,parámetros fisicoquímicos,calidad de agua de mar.

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