Changes in m <sup>6</sup>A RNA methylation are associated with male sterility in wolfberry

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Abstract

Background

N ⁶-methyladenosine (m ⁶A) modification is the most abundant type of RNA modification in eukaryotic cells, playing pivotal roles in multiple plant growth and development processes. Yet the potential role of m ⁶A in conferring the trait of male sterility in plants remains unknown.

Results

In this study, we performed RNA-sequencing (RNA-Seq) and m ⁶A-sequencing (m ⁶A-Seq) of RNAs obtained from the anther tissue of two wolfberry lines: ‘Ningqi No.1’ ( LB1) and its natural male sterile mutant ‘Ningqi No.5’ ( LB5). Based on the newly assembled transcriptome, we established transcriptome-wide m ⁶A maps for LB1 and LB5 at the single nucleus pollen stage. We found that the gene XLOC_021201, a homolog of m ⁶A eraser-related gene ALKBH10 in Arabidopsis thaliana, was significantly differentially expressed between LB1 and LB5. We also identified 1642 and 563 m ⁶A-modified genes with hypermethylated and hypomethylated patterns, respectively, in LB1 compared with LB5. We found the hypermethylated genes significantly enriched in biological processes related to energy metabolism and lipid metabolism, while hypomethylation genes were mainly linked to cell cycle process, gametophyte development, and reproductive process. Among these 2205 differentially m ⁶A methylated genes, 13.74% (303 of 2205) were differentially expressed in LB1 vis-à-vis LB5.

Conclusions

This study constructs the first m ⁶A transcriptome map of wolfberry and establishes an association between m ⁶A and the trait of male sterility in wolfberry.

Supplementary Information

The online version contains supplementary material available at 10.1186/s12870-023-04458-7.

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Author and article information

Contributors

Cuiping Guan: gcp1214@163.com

Chuang Ma: cma@nwafu.edu.cn

Journal

Journal ID (nlm-ta): BMC Plant Biol

Journal ID (iso-abbrev): BMC Plant Biol

Title: BMC Plant Biology

Publisher: BioMed Central (London )

ISSN (Electronic): 1471-2229

Publication date (Electronic): 29 September 2023

Publication date PMC-release: 29 September 2023

Publication date Collection: 2023

Volume: 23

Electronic Location Identifier: 456

Affiliations

[1 ]State Key Laboratory of Crop Stress Biology for Arid Areas, Center of Bioinformatics, College of Life Sciences, Northwest A&F University, ( https://ror.org/0051rme32) Yangling, Shaanxi 712100 China

[2 ]College of Life Science, Ningxia University, ( https://ror.org/04j7b2v61) Yinchuan, Ningxia 750021 China

Article

Publisher ID: 4458

DOI: 10.1186/s12870-023-04458-7

PMC ID: 10540408

PubMed ID: 37770861

SO-VID: 7b1b103b-d813-4530-94b2-bdbab7a11484

License:

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

History

Date received : 12 April 2023

Date accepted : 12 September 2023

Funding

Funded by: Ningxia Natural Science Foundation

Award ID: 2022AAC05020

Award Recipient : Cuiping Guan

Funded by: FundRef http://dx.doi.org/10.13039/501100001809, National Natural Science Foundation of China;

Award ID: 32170681

Award Recipient : Chuang Ma

Funded by: Hundred Talents Program of Shaanxi Province of China

Custom metadata

ScienceOpen disciplines: Plant science & Botany

Keywords: differential methylation,m6a regulators,male sterility,n6-methyladenosine,wolfberry

Data availability:

ScienceOpen disciplines: Plant science & Botany

Keywords: differential methylation, m6a regulators, male sterility, n6-methyladenosine, wolfberry