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      The Transcriptomics of Secondary Growth and Wood Formation in Conifers

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          Abstract

          In the last years, forestry scientists have adapted genomics and next-generation sequencing (NGS) technologies to the search for candidate genes related to the transcriptomics of secondary growth and wood formation in several tree species. Gymnosperms, in particular, the conifers, are ecologically and economically important, namely, for the production of wood and other forestry end products. Until very recently, no whole genome sequencing of a conifer genome was available. Due to the gradual improvement of the NGS technologies and inherent bioinformatics tools, two draft assemblies of the whole genomes sequence of Picea abies and Picea glauca arose in the current year. These draft genome assemblies will bring new insights about the structure, content, and evolution of the conifer genomes. Furthermore, new directions in the forestry, breeding and research of conifers will be discussed in the following. The identification of genes associated with the xylem transcriptome and the knowledge of their regulatory mechanisms will provide less time-consuming breeding cycles and a high accuracy for the selection of traits related to wood production and quality.

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          Reduced growth of Alaskan white spruce in the twentieth century from temperature-induced drought stress.

          The extension of growing season at high northern latitudes seems increasingly clear from satellite observations of vegetation extent and duration. This extension is also thought to explain the observed increase in amplitude of seasonal variations in atmospheric CO2 concentration. Increased plant respiration and photosynthesis both correlate well with increases in temperature this century and are therefore the most probable link between the vegetation and CO2 observations. From these observations, it has been suggested that increases in temperature have stimulated carbon uptake in high latitudes and for the boreal forest system as a whole. Here we present multi-proxy tree-ring data (ring width, maximum late-wood density and carbon-isotope composition) from 20 productive stands of white spruce in the interior of Alaska. The tree-ring records show a strong and consistent relationship over the past 90 years and indicate that, in contrast with earlier predictions, radial growth has decreased with increasing temperature. Our data show that temperature-induced drought stress has disproportionately affected the most rapidly growing white spruce, suggesting that, under recent climate warming, drought may have been an important factor limiting carbon uptake in a large portion of the North American boreal forest. If this limitation in growth due to drought stress is sustained, the future capacity of northern latitudes to sequester carbon may be less than currently expected.
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            Transcriptional networks for lignin biosynthesis: more complex than we thought?

            Lignin is an aromatic heteropolymer and the second most abundant plant biopolymer after cellulose. It is deposited mostly in the secondary cell walls of vascular plants and is essential for water transport, mechanical support and for plant pathogen defense. Lignin biosynthesis is a highly energy-consuming and irreversible process that responds to many developmental and environmental cues, including light, sugar content, circadian clock, plant hormones and wounding. During the past decade, many transcription factors involved in lignin biosynthesis have been identified and characterized. In this review, we assess how these transcriptional activators and repressors modulate lignin biosynthesis, and discuss crosstalk between the lignin biosynthesis pathway and other physiological processes. Copyright © 2010 Elsevier Ltd. All rights reserved.
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              Wood formation in trees.

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                Author and article information

                Journal
                Mol Biol Int
                Mol Biol Int
                MBI
                Molecular Biology International
                Hindawi Publishing Corporation
                2090-2182
                2090-2190
                2013
                29 October 2013
                : 2013
                : 974324
                Affiliations
                1Institute for Biotechnology and Bioengineering, Centre of Genomics and Biotechnology (IBB/CGB), University of Tras-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal
                2Instituto de Investigação Científica Tropical (IICT), Centro de Florestas e Produtos Florestais (FLOR), Tapada da Ajuda, 1349-018 Lisboa, Portugal
                3Department of Forestry Sciences and Landscape (CIFAP), University of Tras-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal
                4Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Tras-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal
                Author notes

                Academic Editor: Joseph Rothnagel

                Article
                10.1155/2013/974324
                3830773
                7a6991c0-ac12-41d6-82fc-a1eac5279401
                Copyright © 2013 Ana Carvalho et al.

                This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

                History
                : 29 April 2013
                : 22 August 2013
                : 9 September 2013
                Categories
                Review Article

                Molecular biology
                Molecular biology

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