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      Delineation of chicken immune markers in the era of omics and multicolor flow cytometry

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          Abstract

          Multiparameter flow cytometry is a routine method in immunological studies incorporated in biomedical, veterinary, agricultural, and wildlife research and routinely used in veterinary clinical laboratories. Its use in the diagnostics of poultry diseases is still limited, but due to the continuous expansion of reagents and cost reductions, this may change in the near future. Although the structure and function of the avian immune system show commonalities with mammals, at the molecular level, there is often low homology across species. The cross-reactivity of mammalian immunological reagents is therefore low, but nevertheless, the list of reagents to study chicken immune cells is increasing. Recent improvement in multicolor antibody panels for chicken cells has resulted in more detailed analysis by flow cytometry and has allowed the discovery of novel leukocyte cell subpopulations. In this article, we present an overview of the reagents and guidance needed to perform multicolor flow cytometry using chicken samples and common pitfalls to avoid.

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          Nomenclature of monocytes and dendritic cells in blood.

          Monocytes and cells of the dendritic cell lineage circulate in blood and eventually migrate into tissue where they further mature and serve various functions, most notably in immune defense. Over recent years these cells have been characterized in detail with the use of cell surface markers and flow cytometry, and subpopulations have been described. The present document proposes a nomenclature for these cells and defines 3 types of monocytes (classical, intermediate, and nonclassical monocytes) and 3 types of dendritic cells (plasmacytoid and 2 types of myeloid dendritic cells) in human and in mouse blood. This classification has been approved by the Nomenclature Committee of the International Union of Immunological Societies, and we are convinced that it will facilitate communication among experts and in the wider scientific community.
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            Multiplexed quantification of proteins and transcripts in single cells

            High-throughput quantification of protein and gene expression in single cells is achieved using DNA-barcoded antibodies.
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              Cross-Species Single-Cell Analysis Reveals Divergence of the Primate Microglia Program

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                Author and article information

                Contributors
                URI : https://loop.frontiersin.org/people/463287/overviewRole: Role: Role: Role: Role: Role:
                URI : https://loop.frontiersin.org/people/723205/overviewRole: Role: Role:
                URI : https://loop.frontiersin.org/people/469822/overviewRole: Role: Role: Role: Role:
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                Journal
                Front Vet Sci
                Front Vet Sci
                Front. Vet. Sci.
                Frontiers in Veterinary Science
                Frontiers Media S.A.
                2297-1769
                23 May 2024
                2024
                : 11
                : 1385400
                Affiliations
                [1] 1Department of Veterinary Sciences, LMU Munich , Munich, Germany
                [2] 2Division of Immunology, The Roslin Institute, Royal (Dick) School of Veterinary Studies, University of Edinburgh , Edinburgh, United Kingdom
                [3] 3Department of Animal and Veterinary Sciences, Aarhus University , Tjele, Denmark
                Author notes

                Edited by: Francisco Javier Salguero, UK Health Security Agency (UKHSA), United Kingdom

                Reviewed by: Roberta Salaroli, University of Bologna, Italy

                Maria Carmela Scata', Council for Agricultural and Economics Research (CREA), Italy

                *Correspondence: Sonja Härtle, Sonja.Haertle@ 123456lmu.de

                Present address: Lonneke Vervelde, Royal GD Animal Health, Deventer, Netherlands

                These authors have contributed equally to this work

                Article
                10.3389/fvets.2024.1385400
                11156169
                38846783
                773bd565-9aa2-4b68-b721-783c28802d0e
                Copyright © 2024 Härtle, Sutton, Vervelde and Dalgaard.

                This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

                History
                : 12 February 2024
                : 02 May 2024
                Page count
                Figures: 6, Tables: 1, Equations: 0, References: 178, Pages: 19, Words: 16276
                Funding
                Funded by: European Union’s Horizon 2020 Research and Innovation Programme
                Funded by: Biotechnology and Biological Sciences Research Council, doi 10.13039/501100000268;
                The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. TD and LV received funding from the European Union’s Horizon 2020 Research and Innovation Programme under grant agreement No. 731014 (VetBioNet—Veterinary Biocontained Facility Network). LV and KS received funding from the Biotechnology and Biological Sciences Research Council under project numbers BBS/E/D/10002071 and BBS/E/D/20002174. SH received funding from the Deutsche Forschungsgemeinschaft in the framework of the Research Unit ImmunoChick (FOR5130) through the project HA-8037/2–1. We would like to thank the British Society of Immunology and the Comparative and Veterinary Immunology group for their funding and contributions to the organization of the Avian Flow Cytometry Workshop.
                Categories
                Veterinary Science
                Review
                Custom metadata
                Veterinary Experimental and Diagnostic Pathology

                chicken,leukocytes,flow cytometry,lineages,phenotyping,single-cell biology

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