For Publishers
DiscoveryMetadataPeer reviewHostingPublishing
For Researchers
JoinPublishReviewCollect
Register
Blog
About
Search
Advanced search
My ScienceOpen
Search
For Publishers
For Researchers
Blog
About
65
views
137
references
 Top references
cited by
110
    
0 reviews
 Review
0
comments
 Comment
0
recommends
+1 Recommend
0 collections
    0
    shares
      780
      similar
       All similar
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      G-quadruplexes and helicases

      research-article

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Guanine-rich DNA strands can fold in vitro into non-canonical DNA structures called G-quadruplexes. These structures may be very stable under physiological conditions. Evidence suggests that G-quadruplex structures may act as ‘knots’ within genomic DNA, and it has been hypothesized that proteins may have evolved to remove these structures. The first indication of how G-quadruplex structures could be unfolded enzymatically came in the late 1990s with reports that some well-known duplex DNA helicases resolved these structures in vitro. Since then, the number of studies reporting G-quadruplex DNA unfolding by helicase enzymes has rapidly increased. The present review aims to present a general overview of the helicase/G-quadruplex field.

          Related collections

          Most cited references137

          • Record: found
          • Abstract: found
          • Article: not found

          Structure and mechanism of helicases and nucleic acid translocases.

          Martin R. Singleton, Mark S Dillingham, Dale B Wigley (2007)
          Helicases and translocases are a ubiquitous, highly diverse group of proteins that perform an extraordinary variety of functions in cells. Consequently, this review sets out to define a nomenclature for these enzymes based on current knowledge of sequence, structure, and mechanism. Using previous definitions of helicase families as a basis, we delineate six superfamilies of enzymes, with examples of crystal structures where available, and discuss these structures in the context of biochemical data to outline our present understanding of helicase and translocase activity. As a result, each superfamily is subdivided, where appropriate, on the basis of mechanistic understanding, which we hope will provide a framework for classification of new superfamily members as they are discovered and characterized.
          Article 0 comments Cited 437 times – based on 0 reviews     Review now
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            The Bloom's syndrome gene product is homologous to RecQ helicases.

            N A Ellis, J Groden, T Ye … (1995)
            The Bloom's syndrome (BS) gene, BLM, plays an important role in the maintenance of genomic stability in somatic cells. A candidate for BLM was identified by direct selection of a cDNA derived from a 250 kb segment of the genome to which BLM had been assigned by somatic crossover point mapping. In this novel mapping method, cells were used from persons with BS that had undergone intragenic recombination within BLM. cDNA analysis of the candidate gene identified a 4437 bp cDNA that encodes a 1417 amino acid peptide with homology to the RecQ helicases, a subfamily of DExH box-containing DNA and RNA helicases. The presence of chain-terminating mutations in the candidate gene in persons with BS proved that it was BLM.
            Article 0 comments Cited 261 times – based on 0 reviews     Review now
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              DNA replication through G-quadruplex motifs is promoted by the Saccharomyces cerevisiae Pif1 DNA helicase.

              Katrin Paeschke, John A Capra, Virginia Zakian (2011)
              G-quadruplex (G4) DNA structures are extremely stable four-stranded secondary structures held together by noncanonical G-G base pairs. Genome-wide chromatin immunoprecipitation was used to determine the in vivo binding sites of the multifunctional Saccharomyces cerevisiae Pif1 DNA helicase, a potent unwinder of G4 structures in vitro. G4 motifs were a significant subset of the high-confidence Pif1-binding sites. Replication slowed in the vicinity of these motifs, and they were prone to breakage in Pif1-deficient cells, whereas non-G4 Pif1-binding sites did not show this behavior. Introducing many copies of G4 motifs caused slow growth in replication-stressed Pif1-deficient cells, which was relieved by spontaneous mutations that eliminated their ability to form G4 structures, bind Pif1, slow DNA replication, and stimulate DNA breakage. These data suggest that G4 structures form in vivo and that they are resolved by Pif1 to prevent replication fork stalling and DNA breakage. Copyright © 2011 Elsevier Inc. All rights reserved.
              Article 0 comments Cited 242 times – based on 0 reviews     Review now
                Bookmark
                All references

                Author and article information

                Journal
                Journal ID (nlm-ta): Nucleic Acids Res
                Journal ID (iso-abbrev): Nucleic Acids Res
                Journal ID (hwp): nar
                Journal ID (publisher-id): nar
                Title: Nucleic Acids Research
                Publisher: Oxford University Press
                ISSN (Print): 0305-1048
                ISSN (Electronic): 1362-4962
                Publication date (Print): 18 March 2016
                Publication date (Electronic): 15 February 2016
                Publication date PMC-release: 15 February 2016
                Volume: 44
                Issue: 5
                Pages: 1989-2006
                Affiliations
                [1 ]University of Bordeaux, ARNA Laboratory F-33000 Bordeaux, France
                [2 ]INSERM U1212,CNRS UMR 5320, IECB, F-33600 Pessac, France
                [3 ]CNRS UMR 7196, INSERM U1154, MNHN, F-75005 Paris, France
                [4 ]Sorbonne Universités, F-75005 Paris, France
                [5 ]Laboratory of Molecular Gerontology, National Institute on Aging, NIH, Baltimore, MD 21224, USA
                Author notes
                [* ]To whom correspondence should be addressed. Tel: +33 5 4000 3022; Email: jean-louis.mergny@ 123456inserm.fr
                Correspondence may also be addressed to Jean-Baptiste Boulé. Tel: +33 1 40 79 56 16; Email: jean-baptiste.boule@ 123456mnhn.fr
                Correspondence may also be addressed to Robert M. Brosh Jr. Tel: +1 410 558 8578; Email: broshr@ 123456grc.nia.nih.gov
                Article
                DOI: 10.1093/nar/gkw079
                PMC ID: 4797304
                PubMed ID: 26883636
                SO-VID: 768df57a-0354-4d79-b3a3-a2fb2a7b2b47
                Copyright © © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.
                License:

                This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@ 123456oup.com

                History
                Date accepted : 01 February 2016
                Date revision received : 26 January 2016
                Date received : 24 November 2015
                Page count
                Pages: 18
                Categories
                Subject: Survey and Summary
                Custom metadata
                cover-date 18 March 2016

                ScienceOpen disciplines: Genetics
                Data availability:
                ScienceOpen disciplines: Genetics

                Comments

                Comment on this article

                scite_

                Similar content780

                See all similar

                Cited by172

                See all cited by

                Most referenced authors2,002

                See all reference authors