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      Gene identification and characterization of fucoidan deacetylase for potential application to fucoidan degradation and diversification.

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          Abstract

          Fucoidan is an α-l-fucopyranosyl polymer found in seaweeds with forms that have acetyl and sulfuric modifications and derivatives that are lower and/or diversified, with modifications that have attracted interest as potential bioactive substances. We identified the gene for a fucoidan deacetylase that cleaves acetyl moieties from fucoidan and thereby contributes to fucoidan utilization in the marine bacterium Luteolibacter algae H18. Fucoidan deacetylase was purified to homogeneity from a cell-free extract of L. algae H18, and used to determine the internal amino acid sequence and identify the gene, fud, in a draft genome sequence of the H18 strain. The gene product was heterologously produced in Escherichia coli and was demonstrated to catalyze fucoidan deacetylation, but not desulfation, and degradation into lower forms. In addition to fucoidan deacetylation, the enzyme catalyzed the hydrolysis of p-nitrophenyl esters with organic acids, and p-nitrophenyl acetate was the best substrate among those tested. The present study provides a new tool for fucoidan degradation, potentially expanding investigations on fucoidan derivatives.

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          Author and article information

          Journal
          J Biosci Bioeng
          Journal of bioscience and bioengineering
          Elsevier BV
          1347-4421
          1347-4421
          Sep 2017
          : 124
          : 3
          Affiliations
          [1 ] Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, Tottori 680-8552, Japan; FUKAEKASEI Co., Ltd., 2-2-7 Murotani, Nishi-ku, Kobe 651-2241, Japan.
          [2 ] Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, Tottori 680-8552, Japan.
          [3 ] Center for Research on Green Sustainable Chemistry, Faculty of Engineering, Tottori University, Tottori 680-8552, Japan.
          [4 ] Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, Tottori 680-8552, Japan. Electronic address: ohshiro@bio.tottori-u.ac.jp.
          Article
          S1389-1723(17)30075-0
          10.1016/j.jbiosc.2017.04.002
          28442389
          754ee55d-e691-4fc0-a80c-8793aff377f5
          Copyright © 2017 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.
          History

          Cladosiphon okamuranus (Okinawamozuku),Deacetylase,Esterase,Fucoidan,Luteolibacter

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