Optimization of carotenoids production by  Rhodotorula mucilaginosa  (MTCC-1403) using agro-industrial waste in bioreactor: A statistical approach

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Graphical abstract

Highlights

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Agro wastes were used as carbon, nitrogen source to produce pigments (Carotenoids) by Rhodotorula mucilaginosa.
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Response Surface Methodology was used to optimize the pH, temperature and agitation in shake flask.
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Impact of aeration was studied in Fermenter.
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LC-MS confirmed the extracted pigment was mixture of Phytoene, Toluene, Torularhodin, and β-carotene.

Abstract

Bio-colorants are preferred over synthetic colors as bio-colorants not only impart characteristic color to the food also contain harmless bio-active antioxidant nutrients. The present study was undertaken to investigate the potential of agro-industrial waste (Onion peels, potato skin, mung bean husk and pea pods) for carotenoid production from Rhodotorula mucilaginosa. After screening of appropriate carbon, nitrogen sources from agro-industrial waste, the fermentation conditions (pH, temperature, agitation) were optimized using Response Surface Methodology and optimum conditions were pH 6.1, temperature 25.8 ᴼC and agitation 119.6 rpm. Further, to evaluate the effect of aeration on carotenoids synthesis, fermentation was carried out in 3 L bio-reactor under optimum conditions with an air input of 1.0 vvm. Aeration causes elevation of more than 100 μg carotenoids per g of dry biomass. LC-MS of extracted pigment confirmed the presence of some other carotenoids along with β-carotene. The major carotenoid compounds were found from the investigation were torularhodin, β-carotene, and torulene.

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Most cited references 24

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Outdoor cultivation of microalgae for carotenoid production: current state and perspectives.

José A Del Campo, Mercedes García-González, Miguel G Guerrero (2007)

Microalgae are a major natural source for a vast array of valuable compounds, including a diversity of pigments, for which these photosynthetic microorganisms represent an almost exclusive biological resource. Yellow, orange, and red carotenoids have an industrial use in food products and cosmetics as vitamin supplements and health food products and as feed additives for poultry, livestock, fish, and crustaceans. The growing worldwide market value of carotenoids is projected to reach over US$1,000 million by the end of the decade. The nutraceutical boom has also integrated carotenoids mainly on the claim of their proven antioxidant properties. Recently established benefits in human health open new uses for some carotenoids, especially lutein, an effective agent for the prevention and treatment of a variety of degenerative diseases. Consumers' demand for natural products favors development of pigments from biological sources, thus increasing opportunities for microalgae. The biotechnology of microalgae has gained considerable progress and relevance in recent decades, with carotenoid production representing one of its most successful domains. In this paper, we review the most relevant features of microalgal biotechnology related to the production of different carotenoids outdoors, with a main focus on beta-carotene from Dunaliella, astaxanthin from Haematococcus, and lutein from chlorophycean strains. We compare the current state of the corresponding production technologies, based on either open-pond systems or closed photobioreactors. The potential of scientific and technological advances for improvements in yield and reduction in production costs for carotenoids from microalgae is also discussed.