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      The acute effects of a multi-ingredient pre-workout supplement on resting energy expenditure and exercise performance in recreationally active females

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          Abstract

          Background

          The use of dietary supplements to improve performance is becoming increasingly popular among athletes and fitness enthusiasts. Unfortunately, there is a tremendous lack of research being done regarding female athletes and the use of sport supplements. The purpose of this study was to examine the acute effects of multi-ingredient pre-workout supplement (MIPS) ingestion on resting metabolism and exercise performance in recreationally-active females.

          Methods

          Fifteen recreationally-active females participated in a randomized, double-blind, placebo controlled study. Subjects completed baseline, and two experimental testing sessions in a cross-over design fashion. Experimental testing included assessment of resting energy expenditure (REE), heart rate, and blood pressure following the ingestion of a MIPS or placebo. Subjects also completed a repetition to failure test for the back squat (BS) and bench press (BP) at 85% of their 5-repetition maximum followed by the assessment of anaerobic power using a counter-movement vertical jump test and a sprint test on a force-treadmill. Subjective measurements of energy, focus, and fatigue were also assessed using a 5-point Likert scale. Separate repeated measures analysis of variance (ANOVA) were used to assess differences in REE, cardiovascular responses, and subjective markers between conditions. Performance data were analyzed using paired Student’s T-tests.

          Results

          A significant main effect for condition was observed for REE ( p = 0.021) and diastolic blood pressure ( p = 0.011) following ingestion of the MIPS. The supplement condition resulted in a greater number of BP repetitions to failure and total work completed during treadmill test ( p = 0.039) compared to placebo ( p = 0.037). A significant condition x time interaction for focus was observed with the supplement treatment exhibiting improved focus at 80-min post ingestion ( p = 0.046).

          Conclusions

          Consumption of a MIPS increased resting metabolism following a single dose accompanied by an increase in diastolic blood pressure. Furthermore, acute MIPS ingestion improved upper body muscular endurance and anaerobic capacity while improving feelings of focus following high-intensity exercise in recreationally active females.

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          Most cited references29

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          International society of sports nutrition position stand: caffeine and performance

          Position Statement: The position of The Society regarding caffeine supplementation and sport performance is summarized by the following seven points: 1.) Caffeine is effective for enhancing sport performance in trained athletes when consumed in low-to-moderate dosages (~3-6 mg/kg) and overall does not result in further enhancement in performance when consumed in higher dosages (≥ 9 mg/kg). 2.) Caffeine exerts a greater ergogenic effect when consumed in an anhydrous state as compared to coffee. 3.) It has been shown that caffeine can enhance vigilance during bouts of extended exhaustive exercise, as well as periods of sustained sleep deprivation. 4.) Caffeine is ergogenic for sustained maximal endurance exercise, and has been shown to be highly effective for time-trial performance. 5.) Caffeine supplementation is beneficial for high-intensity exercise, including team sports such as soccer and rugby, both of which are categorized by intermittent activity within a period of prolonged duration. 6.) The literature is equivocal when considering the effects of caffeine supplementation on strength-power performance, and additional research in this area is warranted. 7.) The scientific literature does not support caffeine-induced diuresis during exercise, or any harmful change in fluid balance that would negatively affect performance.
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            Nitrate supplementation's improvement of 10-km time-trial performance in trained cyclists.

            Six days of dietary nitrate supplementation in the form of beetroot juice (~0.5 L/d) has been reported to reduce pulmonary oxygen uptake (VO₂) during submaximal exercise and increase tolerance of high-intensity work rates, suggesting that nitrate can be a potent ergogenic aid. Limited data are available regarding the effect of nitrate ingestion on athletic performance, and no study has investigated the potential ergogenic effects of a small-volume, concentrated dose of beetroot juice. The authors tested the hypothesis that 6 d of nitrate ingestion would improve time-trial performance in trained cyclists. Using a double-blind, repeated-measures crossover design, 12 male cyclists (31±3 yr, VO2peak=58±2 ml·kg⁻¹·min⁻¹, maximal power [Wmax]=342±10 W) ingested 140 ml/d of concentrated beetroot (~8 mmol/d nitrate) juice (BEET) or a placebo (nitrate-depleted beetroot juice; PLAC) for 6 d, separated by a 14-d washout. After supplementation on Day 6, subjects performed 60 min of submaximal cycling (2×30 min at 45% and 65% Wmax, respectively), followed by a 10-km time trial. Time-trial performance (953±18 vs. 965±18 s, p<.005) and power output (294±12 vs. 288±12 W, p<.05) improved after BEET compared with PLAC supplementation. Submaximal VO₂ was lower after BEET (45% Wmax=1.92±0.06 vs. 2.02±0.09 L/min, 65% Wmax 2.94±0.12 vs. 3.11±0.12 L/min) than with PLAC (main effect, p<.05). Whole-body fuel selection and plasma lactate, glucose, and insulin concentrations did not differ between treatments. Six days of nitrate supplementation reduced VO₂ during submaximal exercise and improved time-trial performance in trained cyclists.
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              Effect of caffeine ingestion on muscular strength and endurance: a meta-analysis.

              Our objective was to perform a systematic review and meta-analysis of the research literature assessing the effect of caffeine ingestion on maximal voluntary contraction (MVC) strength and muscular endurance. Thirty-four relevant studies between 1939 and 2008 were included in the meta-analyses of caffeine's effects on MVC strength (n = 27 studies) and muscular endurance (n = 23 studies). Effect sizes (ES) were calculated as the standardized mean difference and meta-analyses were completed using a random-effects model. Overall, caffeine ingestion was found to result in a small beneficial effect on MVC strength (overall ES = 0.19, P = 0.0003). However, caffeine appears to improve MVC strength primarily in the knee extensors (i.e., by approximately 7%, ES = 0.37) and not in other muscle groups such as the forearm or the knee flexors. In an attempt to offer a physiological mechanism behind caffeine's ability to improve MVC strength, a meta-analysis was run on ES from nine studies that measured percent muscle activation during MVC in trials comparing caffeine versus placebo; the overall ES (0.67) was highly significant (P = 0.00008) and of moderate to large size, thus implicating an effect of caffeine on the CNS. Caffeine ingestion was also found to exert a small beneficial effect on muscular endurance (overall ES = 0.28, P = 0.00005). However, it appears caffeine improves muscular endurance only when it is assessed using open (i.e., by approximately 18%, ES = 0.37) and not fixed end point tests. Overall, caffeine ingestion improves MVC strength and muscular endurance. The effect on strength appears exclusively in the knee extensors, and the effect on muscular endurance appears only detectable with open end point tests.
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                Author and article information

                Contributors
                michael.cameron@mnsu.edu
                ccamic1@niu.edu
                sdoberstein@uwlax.edu
                erickson.jacob@mayo.edu
                636-949-4779 , ajagim@lindenwood.edu
                Journal
                J Int Soc Sports Nutr
                J Int Soc Sports Nutr
                Journal of the International Society of Sports Nutrition
                BioMed Central (London )
                1550-2783
                5 January 2018
                5 January 2018
                2018
                : 15
                : 1
                Affiliations
                [1 ]ISNI 0000 0001 2169 5137, GRID grid.267462.3, Exercise & Sport Science Department, , University of Wisconsin – La Crosse, ; La Crosse, WI USA
                [2 ]ISNI 0000 0000 9003 8934, GRID grid.261128.e, Department of Kinesiology and Physical Education, , Northern Illinois University, ; DeKalb, IL 60115 USA
                [3 ]Mayo Clinic Health Systems, Sports Medicine, Onalaska, WI 54650 USA
                [4 ]ISNI 0000 0000 8539 0749, GRID grid.431378.a, Department of Exercise Science, , Lindenwood University, ; St. Charles, MO 63301 USA
                Author information
                http://orcid.org/0000-0002-6651-5096
                Article
                206
                10.1186/s12970-017-0206-7
                5755346
                29311763
                73ebc6be-4eeb-4015-a0ed-08542cbf3b7f
                © The Author(s) 2018

                Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License ( http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

                History
                : 1 September 2017
                : 26 December 2017
                Funding
                Funded by: MusclePharm
                Award ID: NA
                Funded by: International Society of Sports Nutrition
                Award ID: NA
                Categories
                Research Article
                Custom metadata
                © The Author(s) 2018

                Sports medicine
                thermogenic,ergogenic aid,fatigue,muscular endurance
                Sports medicine
                thermogenic, ergogenic aid, fatigue, muscular endurance

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