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      Fast, inexpensive, and reliable HPLC method to determine monomer fractions in poly(3-hydroxybutyrate-co-3-hydroxyvalerate)

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          Abstract

          The determination of the monomer fractions in polyhydroxyalkanoates is of great importance for research on microbial-produced plastic material. The development of new process designs, the validation of mathematical models, and intelligent control strategies for production depend enormously on the correctness of the analyzed monomer fractions. Most of the available detection methods focus on the determination of the monomer fractions of the homopolymer poly(3-hydroxybutyrate). Only a few can analyze the monomer content in copolymers such as poly(3-hydroxybutyrate-co-3-hydroxyvalerate), which usually require expensive measuring devices, a high preparation time or the use of environmentally harmful halogenated solvents such as chloroform or dichloromethane. This work presents a fast, simple, and inexpensive method for the analysis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with high-performance liquid chromatography. Samples from a bioreactor experiment for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with Cupriavidus necator H16 were examined regarding their monomer content using the new method and gas chromatography analysis, one of the most frequently used methods in literature. The results from our new method were validated using gas chromatography measurements and show excellent agreement.

          Key points

          The presented HPLC method is an inexpensive, fast and environmentally friendly alternative to existing methods for quantification of monomeric composition of PHBV.

          Validation with state of the art GC measurement exhibits excellent agreement over a broad range of PHBV monomer fractions.

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          Marine pollution. Plastic waste inputs from land into the ocean.

          Plastic debris in the marine environment is widely documented, but the quantity of plastic entering the ocean from waste generated on land is unknown. By linking worldwide data on solid waste, population density, and economic status, we estimated the mass of land-based plastic waste entering the ocean. We calculate that 275 million metric tons (MT) of plastic waste was generated in 192 coastal countries in 2010, with 4.8 to 12.7 million MT entering the ocean. Population size and the quality of waste management systems largely determine which countries contribute the greatest mass of uncaptured waste available to become plastic marine debris. Without waste management infrastructure improvements, the cumulative quantity of plastic waste available to enter the ocean from land is predicted to increase by an order of magnitude by 2025.
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            Start a Research on Biopolymer Polyhydroxyalkanoate (PHA): A Review

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              A rapid gas chromatographic method for the determination of poly-?-hydroxybutyric acid in microbial biomass

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                Author and article information

                Contributors
                stefanie.duvigneau@ovgu.de
                Journal
                Appl Microbiol Biotechnol
                Appl Microbiol Biotechnol
                Applied Microbiology and Biotechnology
                Springer Berlin Heidelberg (Berlin/Heidelberg )
                0175-7598
                1432-0614
                20 May 2021
                20 May 2021
                2021
                : 105
                : 11
                : 4743-4749
                Affiliations
                [1 ]GRID grid.5807.a, ISNI 0000 0001 1018 4307, Institute for Automation Engineering, , Otto von Guericke University, ; Magdeburg, Saxony-Anhalt Germany
                [2 ]GRID grid.427932.9, ISNI 0000 0001 0692 3664, Department of Applied Biosciences and Process Technology, , Anhalt University of Applied Sciences, ; Köthen, Saxony-Anhalt Germany
                [3 ]GRID grid.419517.f, ISNI 0000 0004 0491 802X, Process Synthesis and Process Dynamics, , Max Planck Institute for Dynamics of Complex Technical Systems, ; Magdeburg, Saxony-Anhalt Germany
                Author information
                http://orcid.org/0000-0002-7829-5190
                Article
                11265
                10.1007/s00253-021-11265-3
                8195778
                34014345
                72d1ff46-a71e-4f54-aca2-e9365e10d1a9
                © The Author(s) 2021

                Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

                History
                : 5 November 2020
                : 26 March 2021
                : 4 April 2021
                Funding
                Funded by: EU-EFRE Sachsen-Anhalt
                Award ID: DIGIPOL
                Award ID: MAPO
                Award Recipient :
                Funded by: Otto-von-Guericke-Universität Magdeburg (3121)
                Categories
                Methods and Protocols
                Custom metadata
                © Springer-Verlag GmbH Germany, part of Springer Nature 2021

                Biotechnology
                bioplastic,polyhydroxyalkanoate,quantitative measurement,high-pressure liquid chromatography,uv/vis spectrometry

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