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      Giant Magnetoresistance-based Biosensor for Detection of Influenza A Virus

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          Abstract

          We have developed a simple and sensitive method for the detection of influenza A virus based on giant magnetoresistance (GMR) biosensor. This assay employs monoclonal antibodies to viral nucleoprotein (NP) in combination with magnetic nanoparticles (MNPs). Presence of influenza virus allows the binding of MNPs to the GMR sensor and the binding is proportional to the concentration of virus. Binding of MNPs onto the GMR sensor causes change in the resistance of sensor, which is measured in a real time electrical readout. GMR biosensor detected as low as 1.5 × 10 2 TCID 50/mL virus and the signal intensity increased with increasing concentration of virus up to 1.0 × 10 5 TCID 50/mL. This study showed that the GMR biosensor assay is relevant for diagnostic application since the virus concentration in nasal samples of influenza virus infected swine was reported to be in the range of 10 3 to 10 5 TCID 50/mL.

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          Giant Magnetoresistance of (001)Fe/(001)Cr Magnetic Superlattices

          M. N. Baibich, J. M. Broto, A Fert … (1988)
          Article 0 comments Cited 1947 times – based on 0 reviews     Review now
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            Enhanced magnetoresistance in layered magnetic structures with antiferromagnetic interlayer exchange

            G. Binasch, P. Grünberg, F. Saurenbach … (1989)
            Article 0 comments Cited 943 times – based on 0 reviews     Review now
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              Magnetoresistive-based biosensors and biochips.

              Luisa Ferreira, Stuart Graham, Paulo P. Freitas (2004)
              Over the past five years, magnetoelectronics has emerged as a promising new platform technology for biosensor and biochip development. The techniques are based on the detection of the magnetic fringe field of a magnetically labeled biomolecule interacting with a complementary biomolecule bound to a magnetic-field sensor. Magnetoresistive-based sensors, conventionally used as read heads in hard disk drives, have been used in combination with biologically functionalized magnetic labels to demonstrate the detection of molecular recognition. Real-world bio-applications are now being investigated, enabling tailored device design, based on sensor and label characteristics. This detection platform provides a robust, inexpensive sensing technique with high sensitivity and considerable scope for quantitative signal data, enabling magnetoresistive biochips to meet specific diagnostic needs that are not met by existing technologies.
              Article 0 comments Cited 102 times – based on 0 reviews     Review now
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                Author and article information

                Contributors
                Journal
                Journal ID (nlm-ta): Front Microbiol
                Journal ID (iso-abbrev): Front Microbiol
                Journal ID (publisher-id): Front. Microbiol.
                Title: Frontiers in Microbiology
                Publisher: Frontiers Media S.A.
                ISSN (Electronic): 1664-302X
                Publication date (Electronic): 29 March 2016
                Publication date Collection: 2016
                Volume: 7
                Electronic Location Identifier: 400
                Affiliations
                [1] 1Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul MN, USA
                [2] 2Department of Electrical and Computer Engineering, University of Minnesota, Minneapolis MN, USA
                Author notes

                Edited by: Fabrice Merien, Auckland University of Technology, New Zealand

                Reviewed by: Bernard A. P. Lafont, National Institutes of Health, USA; Benoit Chassaing, Georgia State University, USA

                *Correspondence: Jian-Ping Wang, jpwang@ 123456umn.edu ; Andres M. Perez, aperez@ 123456umn.edu

                † These authors have contributed equally to this work.

                This article was submitted to Infectious Diseases, a section of the journal Frontiers in Microbiology

                Article
                DOI: 10.3389/fmicb.2016.00400
                PMC ID: 4809872
                PubMed ID: 27065967
                SO-VID: 71e9611d-753e-424b-8ef7-d41bd6a4e6ff
                Copyright © Copyright © 2016 Krishna, Wu, Perez and Wang.
                License:

                This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

                History
                Date received : 18 January 2016
                Date accepted : 14 March 2016
                Page count
                Figures: 5, Tables: 0, Equations: 0, References: 41, Pages: 8, Words: 0
                Categories
                Subject: Microbiology
                Subject: Methods

                ScienceOpen disciplines: Microbiology & Virology
                Keywords: giant magnetoresistance,biosensor,magnetic nanoparticle,gmr chip,influenza a virus
                Data availability:
                ScienceOpen disciplines: Microbiology & Virology
                Keywords: giant magnetoresistance, biosensor, magnetic nanoparticle, gmr chip, influenza a virus

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