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      Repelencia de adultos de mosca blanca Trialeurodes vaporariorum (Heteroptera: Aleyrodidae) con clavo y pimienta Translated title: Repellency of adults of whitefly Trialeurodes vaporariorum (Heteroptera: Aleyrodidae) with clove and pipper

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          Abstract

          Resumen En la mosca blanca Trialeurodes vaporariorum, plaga de importancia económica en hortalizas, su manejo con insecticidas sintéticos ha generado resistencia con efectos adversos para el ambiente. En la búsqueda de alternativas de manejo, se planteó esta investigación con el objetivo de cuantificar la repelencia de adultos con extractos crudos (metanol, etanol, diclorometano y hexano), aceites esenciales (AE) y compuestos mayoritarios de clavo y pimienta. En un vaso con 10 mL de agua, se colocó una hoja de tomate tratada a concentraciones de 1,0 a 0,000001 % y se introdujeron 20 adultos de 2 d de edad. Luego, de las 3 a las 72 h, se cuantificaron los adultos posados y por diferencia se determinó la repelencia. Los extractos crudos, AE y compuestos mayoritarios de clavo y pimienta repelieron entre 32,5 y 90,2 % la población de adultos de las 3 a las 72 h. Los AE de clavo y pimienta presentan mejor efecto repelente que los extractos crudos y compuestos secundarios, ya que lograron repeler de 46,2 a 100 %; el AE de clavo a la concentración de 1,0 % logró 100 % de repelencia a las 48 y 72 h y el AE de pimienta alcanza 97,5 % a las 72 h. En la práctica, se debe utilizar extracto crudo etanólico de ambas plantas a la concentración de 0,000001 % ya que logran repeler entre 38,7 y 87,5 % de adultos, de las 3 a las 72 h, y su efecto repelente persiste; además, es más fácil de conseguir, más seguro para el operador, distribuidor y consumidor; y está considerado en las normas de agricultura orgánica como solvente para la extracción de compuestos secundarios.

          Translated abstract

          Abstract The whitefly Trialeurodes vaporariorum, an economically important pest in vegetables, is managed with synthetic pesticides and has generated resistance with adverse effects on the environment. In the search for alternative management, adult repellence with raw extracts (methanol, ethanol, dichloromethano, and hexane) essential oils, and major compounds of clove and pepper was tested. For this, a treated tomato leaf was placed at concentrations of 1.0 to 0.000001 % within a 1 L beaker containing a 10 mL flask with water. Subsecuently, 20 adults of 2 d of age were introduced. After 3 to 72 h, the posed adults were quantified, and repellence was determined from the difference. The raw extracts, essential oils, and major compounds of clove and pepper repel from 32.5 to 90.2 % of the adult population in 3 to 72 h. The cloves and pepper EO have a better repellent effect than raw extracts and secondary compounds, since they manage to repel 46.2 % to 100 %. The clove EO at the concentration of 1.0 % achieves 100 % repellency at 48 and 72 h after application, while the pepper EO reaches 97.5 % at 72 h after application; in practice, ethanolic crude extract of both plants should be used at a concentration of 0.000001 % since they can repel 38.7 to 87.5 % of adults, from 3 to 72 h, and its repellent effect is maintained; also, it is easier to acquire, safer for the operator, distributor and consumer; and is considered a solvent for the extraction of secondary compounds in organic farming standards.

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          Most cited references53

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          Comparison of essential oils of clove buds extracted with supercritical carbon dioxide and other three traditional extraction methods

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            Molecular and pharmacological analysis of an octopamine receptor from American cockroach and fruit fly in response to plant essential oils.

            Octopamine receptors from American cockroach, Periplaneta americana (Pa oa1), and fruit fly, Drosophila melanogaster (OAMB), were cloned and permanently expressed in HEK-293 cells, and found to activate adenylate cyclase activity and increase [Ca2+]i levels through G-protein coupled receptor signaling pathways. Sequencing information (GenBank accession number AY333178) and functional data of Pa oa1 were recently published. Saturation binding analysis with 3H-yohimbine was performed with Pa oa(1) and OAMB expressed in COS-7 cells. The K(d) values were determined to be 28.4 and 43.0 nM, respectively. B(max) was determined to be 11.8 and 8.04 pmol receptor/mg protein, respectively. Competitive binding data using cell membranes expressing either OAMB or Pa oa1 demonstrated significantly decreased binding activity in binding assays performed in the presence of plant essential oils, eugenol, cinnamic alcohol, and trans-anethole. Eugenol decreased cAMP level in HEK-293 cells expressing Pa oa1, but trans-anethole increased cAMP in HEK-293 cells expressing OAMB. All three chemicals increased [Ca2+]i level in both cell models. Toxicity data against fruit flies and American cockroaches demonstrated species differences in response to treatment with tested plant essential oils. The toxicity of tested chemicals against wild type and octopamine mutant (iav) fly strains suggested that an octopamine receptor mediates the toxicity of cinnamic alcohol, eugenol, trans-antehole, and 2-phenethyl propionate against fruit flies. Collectively, the data suggest a correlation between cellular changes induced by tested plant essential oils and their toxicity against fruit fly and American cockroach.
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              Larvicidal property of essential oils against Culex quinquefasciatus Say (Diptera: Culicidae)

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                Author and article information

                Journal
                rcen
                Revista Colombiana de Entomología
                Rev. Colomb. Entomol.
                Sociedad Colombiana de Entomología (Bogotá, Distrito Capital, Colombia )
                0120-0488
                2665-4385
                December 2020
                : 46
                : 2
                : e7520
                Affiliations
                [5] Texcoco orgnameColegio de Postgraduados orgdiv1Posgrado de Fitosanidad Mexico nestor@ 123456colpos.mx
                [4] Texcoco orgnameColegio de Postgraduados orgdiv1Posgrado de Botanica Mexico msoto@ 123456colpos.mx
                [1] Ocozocoautla orgnameUniversidad Autónoma de Chiapas orgdiv1Facultad de Ciencias Agronómicas Mexico guerr2012@ 123456hotmail.es
                [2] Texcoco orgnameColegio de Postgraduados orgdiv1Posgrado de Fitosanidad Mexico crhernan@ 123456colpos.mx
                [6] Ocozocoautla orgnameUniversidad Autónoma de Chiapas orgdiv1Facultad de Ciencias Agronómicas Mexico fragueher@ 123456prodigy.net.mx
                [3] Texcoco orgnameColegio de Postgraduados orgdiv1Posgrado de Fitosanidad Mexico
                Article
                S0120-04882020000200004 S0120-0488(20)04600200004
                10.25100/socolen.v46i2.7520
                71b4e7da-7049-411b-b14d-3ba20d7e30b8

                This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

                History
                : 13 January 2020
                : 14 February 2019
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 53, Pages: 0
                Product

                SciELO Colombia

                Self URI: Texto completo solamente en formato PDF (ES)
                Categories
                Sección agrícola

                Extractos,aceites,compuestos,acuosos,crudos,condimento,plantas aromáticas,Extracts,oils,compound,aqueous,raw,seasoning,aromatic plants

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