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Abstract
In this study, one kind of environmentally friendly solvents named polyethylene glycol
(PEG) was developed for the ultrasound-assisted enzymatic extraction (UAEE) of polysaccharides
from Gingko biloba leaves (GBLP). Box-Behnken design (BBD) was used to optimize the
UAEE conditions of GBLP. Results showed that the optimal extraction conditions were:
a pH of 4.34, an extraction temperature of 51.88 °C and an extraction time of 37.13
min. Under these optimal extraction conditions, the GBLP yield was 7.29±0.21%, which
was well in agreement with the value predicted by the mathematical model.