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      Evaluating the utility of time-lapse imaging in the estimation of post-mortem interval: An Australian case study

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          Abstract

          Estimating post-mortem interval is an important aspect in forensic investigations. The aim of this study was to investigate if time-lapse imaging can be used to improve estimates of post-mortem interval using Megyesi et al.‘s [1] method for a human donor decomposing in an Australian environment. To achieve this, time-lapse images were taken every 30 min over a 6-month period. The Megyesi et al. [1] total body score (TBS) system was used to quantify the level of decomposition of the donor for each image. Linear regression was performed to determine if observing decomposition more than once a day leads to increased accuracy in predicting PMI (post-mortem interval).

          Decomposition initially progressed quickly and then plateaued at 1004 hours PMI, with a TBS of 24. Individual timestamps were created from the time-lapse images taken each day at 08:00 hrs, 11:00 hrs, 14:00 hrs, 15:00 hrs, and 17:00 hrs. All timestamps produced R 2 values > 0.80, indicating that the Megyesi et al. [1] method accurately predicts PMI for this donor. The 08:00 hrs timestamp had the highest value R 2 = 0.886, whilst the combined timestamp (which included the scores from all five images for each 24-hour period) R 2 = 0.823 was the lowest.

          This study supports the validity of Megyesi et al.‘s [1] TBS model to estimate PMI. Two other interesting findings were that the results suggest that scoring TBS multiple times per day does not improve estimates of PMI, however scoring TBS at daybreak produces more accurate results than scoring TBS later in the day. This may be an important consideration in forensic scenarios.

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          Using accumulated degree-days to estimate the postmortem interval from decomposed human remains.

          Forensic anthropologists often rely on the state of decomposition to estimate the postmortem interval (PMI) in a human remains case. The state of decomposition can provide much information about the PMI, especially when decomposition is treated as a semi-continuous variable and used in conjunction with accumulated-degree-days (ADD). This preliminary study demonstrates a supplemental method of determining the PMI based on scoring decomposition using a point-based system and taking into account temperatures in which the remains were exposed. This project was designed to examine the ways that forensic anthropologists could improve their PMI estimates based on decomposition by using a more quantitative approach. A total of 68 human remains cases with a known date of death were scored for decomposition and a regression equation was calculated to predict ADD from decomposition score. ADD accounts for approximately 80% of the variation in decomposition. This study indicates that decomposition is best modeled as dependent on accumulated temperature, not just time.
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            The elusive universal post-mortem interval formula.

            Arpad Vass (2011)
            The following manuscript details our initial attempt at developing universal post-mortem interval formulas describing human decomposition. These formulas are empirically derived from data collected over the last 20 years from the University of Tennessee's Anthropology Research Facility, in Knoxville, Tennessee, USA. Two formulas were developed (surface decomposition and burial decomposition) based on temperature, moisture, and the partial pressure of oxygen, as being three of the four primary drivers for human decomposition. It is hoped that worldwide application of these formulas to environments and situations not readily studied in Tennessee will result in interdisciplinary cooperation between scientists and law enforcement personnel that will allow for future refinements of these models leading to increased accuracy. Copyright © 2010 Elsevier Ireland Ltd. All rights reserved.
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              Human decomposition and the reliability of a 'Universal' model for post mortem interval estimations.

              Human decomposition is a complex biological process driven by an array of variables which are not clearly understood. The medico-legal community have long been searching for a reliable method to establish the post-mortem interval (PMI) for those whose deaths have either been hidden, or gone un-noticed. To date, attempts to develop a PMI estimation method based on the state of the body either at the scene or at autopsy have been unsuccessful. One recent study has proposed that two simple formulae, based on the level of decomposition humidity and temperature, could be used to accurately calculate the PMI for bodies outside, on or under the surface worldwide. This study attempted to validate 'Formula I' [1] (for bodies on the surface) using 42 Canadian cases with known PMIs. The results indicated that bodies exposed to warm temperatures consistently overestimated the known PMI by a large and inconsistent margin for Formula I estimations. And for bodies exposed to cold and freezing temperatures (less than 4°C), then the PMI was dramatically under estimated. The ability of 'Formulae II' to estimate the PMI for buried bodies was also examined using a set of 22 known Canadian burial cases. As these cases used in this study are retrospective, some of the data needed for Formula II was not available. The 4.6 value used in Formula II to represent the standard ratio of time that burial decelerates the rate of decomposition was examined. The average time taken to achieve each stage of decomposition both on, and under the surface was compared for the 118 known cases. It was found that the rate of decomposition was not consistent throughout all stages of decomposition. The rates of autolysis above and below the ground were equivalent with the buried cases staying in a state of putrefaction for a prolonged period of time. It is suggested that differences in temperature extremes and humidity levels between geographic regions may make it impractical to apply formulas developed in one region to any other region. These results also suggest that there are other variables, apart from temperature and humidity that may impact the rate of human decomposition. These variables, or complex of variables, are considered regionally specific. Neither of the Universal Formulae performed well, and our results do not support the proposition of Universality for PMI estimation.
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                Author and article information

                Contributors
                Journal
                Forensic Sci Int
                Forensic Sci Int
                Forensic Science International: Synergy
                Elsevier
                2589-871X
                17 August 2019
                2019
                17 August 2019
                : 1
                : 204-210
                Affiliations
                [a ]School of Health, Medical and Applied Sciences, Central Queensland University, Rockhampton, Queensland, 4700, Australia
                [b ]School of Medical Sciences, University of New South Wales, Sydney, New South Wales, 2052, Australia
                [c ]Centre for Forensic Science, University of Technology Sydney, Ultimo, New South Wales, 2007, Australia
                [d ]School of Community Health, Charles Sturt University, Port Macquarie, New South Wales, 2444, Australia
                [e ]School of Humanities and Social Science, University of Newcastle, Callaghan, New South Wales, 2308, Australia
                [f ]Honorary Associate in Centre for Forensic Science, University of Technology, Sydney, Australia
                Author notes
                []Corresponding author. alyson.s.wilson@ 123456gmail.com
                Article
                S2589-871X(19)30142-1
                10.1016/j.fsisyn.2019.08.003
                7219161
                6f98b549-cb8b-4ae5-9200-e37d810d625e
                © 2019 The Authors

                This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

                History
                : 6 May 2019
                : 15 August 2019
                : 16 August 2019
                Categories
                Interdisciplinary Forensics

                Forensic science
                forensic anthropology,taphonomy,time-death interval,australian facility for taphonomic experimental research

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