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Abstract
We investigated the lethal effect of supercritical carbon dioxide (SC-CO(2); temperature:
65, 70 degrees C, pressure: 80, 100, 120 bar, time: 10-40 min) on Alicyclobacillus
acidoterrestris spores (10(6)-10(7)spores/ml) suspended in apple juice. A. acidoterrestris
spores were completely inactivated by SC-CO(2) to undetectable levels in above 65
degrees C, 100 bar for 40 min and 70 degrees C, 80 bar for 30 min. The SC-CO(2) did
not affect (p>0.05) the pH and Brix of apple juice. In electron microscopic observations,
the surface and internal morphological changes and extraction of intracellular materials
of the treated spores were observed. Our results indicate that SC-CO(2) can effectively
kill A. acidoterrestris spores in apple juice with no changes to the quality of the
juice and also provides a complementary understanding of inactivation action of SC-CO(2).