Towards real-time wide-field fluorescence lifetime imaging of 5-ALA labeled brain tumors with multi-tap CMOS cameras

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Abstract

Fluorescence guided neurosurgery based on 5-aminolevulinic acid (5-ALA) has significantly increased maximal safe resections. Fluorescence lifetime imaging (FLIM) of 5-ALA could further boost this development by its increased sensitivity. However, neurosurgeons require real-time visual feedback which was so far limited in dual-tap CMOS camera based FLIM. By optimizing the number of phase frames required for reconstruction, we here demonstrate real-time 5-ALA FLIM of human high- and low-grade glioma with up to 12 Hz imaging rate over a wide field of view (11.0 x 11.0 mm). Compared to conventional fluorescence imaging, real-time FLIM offers enhanced contrast of weakly fluorescent tissue.

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Author and article information

Journal

Journal ID (nlm-ta): Biomed Opt Express

Journal ID (iso-abbrev): Biomed Opt Express

Journal ID (publisher-id): BOE

Title: Biomedical Optics Express

Publisher: Optical Society of America

ISSN (Electronic): 2156-7085

Publication date (Electronic): 26 February 2020

Publication date Collection: 01 March 2020

Volume: 11

Issue: 3

Pages: 1598-1616

Affiliations

[1 ]Medical University of Vienna, Center for Medical Physics and Biomedical Engineering, 1090 Vienna, Austria

[2 ]Medical University of Vienna, Christian Doppler Laboratory OPTRAMED, 1090 Vienna, Austria

[3 ]PCO AG, Science and Research, 93309 Kelheim, Germany

[4 ]Carl Zeiss Meditec AG, 73447 Oberkochen, Germany

[5 ]General Hospital and Medical University of Vienna, Institute of Neurology, 1090 Vienna, Austria

[6 ]General Hospital and Medical University of Vienna, Department of Neurosurgery, 1090 Vienna, Austria

[7 ]These authors contributed equally to this work

Author notes

[* ] rainer.leitgeb@ 123456meduniwien.ac.at

Author information

David Reichert https://orcid.org/0000-0002-9593-6447

Angelika Unterhuber https://orcid.org/0000-0002-1251-3001

Marco Andreana https://orcid.org/0000-0002-8212-0545

Article

Accession ID: PMC7075617 Pmcid ID: PMC7075617 Pmc-uid ID: 7075617 Publisher ID: 382817

DOI: 10.1364/BOE.382817

PMC ID: 7075617

PubMed ID: 32206431

SO-VID: 6b93bc2f-e8ce-4145-b9a9-3ea59d3caf5e

License:

History

Date received : 13 November 2019

Date revision received : 30 January 2020

Date accepted : 04 February 2020

Funding

Funded by: Carl Zeiss Meditec AG 10.13039/501100002806

Funded by: Bundesministerium für Digitalisierung und Wirtschaftsstandort 10.13039/501100012416

Funded by: Österreichische Nationalstiftung für Forschung, Technologie und Entwicklung 10.13039/100010132

Funded by: H2020 Marie Skłodowska-Curie Actions 10.13039/100010665

Award ID: 721766

Funded by: Österreichischen Akademie der Wissenschaften 10.13039/501100001822

Award ID: 25262

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