Noninvasive Ophthalmic Imaging Measures Retinal Degeneration and Vision Deficits in Ndufs4 ^−/− Mouse Model of Mitochondrial Complex I Deficiency

Abstract

Purpose

To characterize postnatal ocular pathology in a Ndufs4 ^{− / −} mouse model of complex I deficiency using noninvasive retinal imaging and visual testing.

Methods

Ndufs4 ^{− / −} mice and wild-type (WT) littermates were analyzed at 3, 5, and 7 weeks postnatal. Retinal morphology was visualized by optical coherence tomography (OCT). OCT images were analyzed for changes in retinal thickness and reflectivity profiles. Visual function was assessed by electroretinogram (ERG) and optomotor reflex (OMR).

Results

Ndufs4 ^{− / −} animals have normal OCT morphology at weaning and develop inner plexiform layer atrophy over weeks 5 to 7. Outer retinal layers show hyporeflectivity of the external limiting membrane (ELM) and photoreceptor ellipsoid zone (EZ). Retinal function is impaired at 3 weeks, with profound deficits in b-wave, a-wave, and oscillatory potential amplitudes. The b-wave and oscillatory potential implicit times are delayed, but the a-wave implicit time is unaffected. Ndufs4 ^{− / −} animals have normal OMR at 3 weeks and present with increasing acuity and contrast OMR deficits at 5 and 7 weeks. Physiological thinning of inner retinal layers, attenuation of ELM reflectivity, and attenuation of ERG b- and a-wave amplitudes occur in WT C57BL/6 littermates between weeks 3 and 7.

Conclusions

Noninvasive ocular imaging captures early-onset retinal degeneration in Ndufs4 ^{− / −} mice and is a tractable approach for investigating retinal pathology subsequent to complex I deficiency.

Translational Relevance

Ophthalmic imaging captures clinically relevant measures of retinal disease in a fast-progressing mouse model of complex I deficiency consistent with human Leigh syndrome.