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      Antioxidant and Antiproliferative Activities of an Ethylacetate Fraction of Picria Fel-Terrae Lour. Herbs

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          Abstract

          Background:

          Excessive production of oxygen free radicals and imbalance in the mechanisms responsible for antioxidant protection may result in the onset of many diseases including breast cancer.

          Objective:

          To evaluate antiproliferative and antioxidant activity of an ethylacetate fraction (EAF) of Picria fel-terrae Lour. Herbs in the T47D cell line.

          Methods:

          Phenolic and total flavonoid contents in EAF were determined. EAF was tested for cytotoxicity and effects on the cell cycle and apoptosis, as well as antioxidant activity.

          Results:

          EAF was found to contain high levels of phenolic agents (92.88 ± 0.50 mg GAE/g), total flavonoid (84.39 ± 0.07 mg QE/g). The EAF of Picria fel-terrae Lour. herbs was found to have an IC50 of 62.98µg/mL, caused accumulation in G0-G1 and S phase and increased early and late apoptosis. Antioxidant activity in DPPH assays gave an IC50 of 166.90 ± 0.10 µg/mL.

          Conclusions:

          The results reveal that EAF of Picria fel-terrae Lour. herbs has antiproliferative activity and strong antioxidant potential. Further studies are now needed to isolate the responsible antiproliferative and antioxidant components.

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          Most cited references37

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          Screening of plant extracts for antioxidant activity: a comparative study on three testing methods.

          Irina I Koleva, Teris A. van Beek, Jozef P H Linssen … (2002)
          Three methods widely employed in the evaluation of antioxidant activity, namely 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method, static headspace gas chromatography (HS-GC) and beta-carotene bleaching test (BCBT), have been compared with regard to their application in the screening of plant extracts. The strengths and limitations of each method have been illustrated by testing a number of extracts, of differing polarity, from plants of the genus Sideritis, and two known antioxidants (butylated hydroxytoluene and rosmarinic acid). The sample polarity was important for the exhibited activity in the BCBT and HS-GC methods but not for the DPPH method. The complex composition of the extracts and partition phenomena affected their activity in each assay. The value of the BCBT method appears to be limited to less polar samples. Although slow, the HS-GC method is preferable for assessing the antioxidant inhibitory properties on the formation of unwanted secondary volatile products. Being rapid, simple and independent of sample polarity, the DPPH method is very convenient for the quick screening of many samples for radical scavenging activity.
          Article 0 comments Cited 222 times – based on 0 reviews     Review now
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            Determination and Involvement of Aqueous Reducing Compounds in Oxidative Defense Systems of Various Senescing Leaves

            Shimon Meir, Joseph Kanner, Bezalel Akiri … (1995)
            Article 0 comments Cited 106 times – based on 0 reviews     Review now
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              Antioxidant and prooxidant behavior of flavonoids: structure-activity relationships.

              G Cao, E Sofic, David Prior (1996)
              The antioxidant and prooxidant behavior of flavonoids and the related activity-structure relationships were investigated in this study using the oxygen radical absorbance capacity assay. Three different reactive species were used in the assay: 2,2'-azobis(2-amidino-propane) dihydrochloride, a peroxyl radical generator; Cu(2+)-H2O2, mainly a hydroxyl radical generator; and Cu2+, a transition metal. Flavonoids including flavones, isoflavones, and flavanones acted as antioxidants against peroxyl and hydroxyl radicals and served as prooxidants in the presence of Cu2+. Both the antioxidant and the copper-initiated prooxidant activities of a flavonoid depend upon the number of hydroxyl substitutions in its backbone structure, which has neither antioxidant nor prooxidant action. In general, the more hydroxyl substitutions, the stronger the antioxidant and prooxidant activities. The flavonoids that contain multiple hydroxyl substitutions showed antiperoxyl radical activities several times stronger than Trolox, an alpha-to copherol analogue. The single hydroxyl substitution at position 5 provides no activity, whereas the di-OH substitution at 3' and 4' is particularly important to the peroxyl radical absorbing activity of a flavonoid. The conjugation between rings A and B does not affect the antioxidant activity but is very important for the copper-initiated prooxidant action of a flavonoid. The O-methylation of the hydroxyl substitutions inactivates both the antioxidant and the prooxidant activities of the flavonoids.
              Article 0 comments Cited 102 times – based on 0 reviews     Review now
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                Author and article information

                Journal
                Journal ID (nlm-ta): Asian Pac J Cancer Prev
                Journal ID (iso-abbrev): Asian Pac. J. Cancer Prev
                Title: Asian Pacific Journal of Cancer Prevention : APJCP
                Publisher: West Asia Organization for Cancer Prevention (Iran )
                ISSN (Print): 1513-7368
                ISSN (Electronic): 2476-762X
                Publication date (Print): 2017
                Volume: 18
                Issue: 2
                Pages: 399-403
                Affiliations
                [1 ] Department of Pharmaceutical Biology, University of Sumatera Utara, Medan, Indonesia
                [2 ] Department of Pharmaceutical Chemistry, University of Sumatera Utara, Medan, Indonesia
                [3 ] Department of Biology, Faculty of Mathematics and Natural Sciences, University of Sumatera Utara, Medan, Indonesia
                [4 ] Department of Pharmacology, Faculty of Pharmacy, University of Sumatera Utara, Medan, Indonesia
                Author notes
                [* ] For Correspondence: dennysatria@ 123456usu.ac.id
                Article
                Publisher ID: APJCP-18-399
                DOI: 10.22034/APJCP.2017.18.2.399
                PMC ID: 5454734
                PubMed ID: 28345821
                SO-VID: 671c95d1-7245-4de8-9112-d7ef3ee3ecf3
                Copyright © Copyright: © Asian Pacific Journal of Cancer Prevention
                License:

                This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License

                History
                Categories
                Subject: Research Article

                Keywords: antiproliferative,antioxidant,picria fel-terrae lour,ethylacetate fraction
                Data availability:
                Keywords: antiproliferative, antioxidant, picria fel-terrae lour, ethylacetate fraction

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