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Abstract
In order to develop a scaffolding material for tissue regeneration, porous matrices
containing collagen and hyaluronic acid were fabricated by freeze drying at -20 degrees
C, -70 degrees C or -196 degrees C. The fabricated porous membranes were cross-linked
using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) in a range of 1-100 mM
concentrations for enhancing mechanical stability of the composite matrix. Scanning
electron microscope (SEM) views of the matrices demonstrated that the matrices obtained
before cross-linking process had interconnected pores with mean diameters of 40, 90
or 230 microm and porosity of 58-66% according to the freezing temperature, and also
the porous structures after cross-linking process were retained. The swelling test
and IR spectroscopic measurement of different cross-linked membranes were carried
out as a measure of the extent of cross-linking. The swelling behavior of cross-linked
membranes showed no significant differences as cross-linking degree increased. FT-IR
spectra showed the increase of the intensity of the absorbencies at amide bonds (1655,
1546, 1458 cm(-1)) compared to that of CH bond (2930 cm(-1)). In enzymatic degradation
test, EDC treated membranes showed significant enhancement of the resistance to collagenase
activity in comparison with 0.625% glutaraldehyde treated membranes. In cytotoxicity
test using L929 fibroblastic cells, the EDC-cross-linked membranes demonstrated no
significant toxicity.