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      Amplicon metabarcoding data of prokaryotes and eukaryotes present in ‘Kalamata’ table olives packaged under modified atmosphere

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          Abstract

          Evaluation of food microbiome is of major importance since it accounts for the product's organoleptic characteristics and their nutritional value. In this dataset, microbes present in olive samples (‘Kalamata’ variety) stored under modified atmosphere and throughout different time-points of the shelf life of the product are presented, originated after 16S and 18S rRNA sequencing. The different time-points analyzed were: T0 (immediately after packaging), T6 (six months of storage), T12 (12 months of storage) and T18 (six months after the end of shelf life). Sequencing was performed on a MiSeq platform with the MiSeq Reagent Kit v3 (600 cycles). The raw sequence data used for analysis are available in NCBI under the Sequence Read Archive (SRA), with BioProject ID PRJNA688686. Raw reads were analyzed using the QIIME2 pipeline, clustered into Operational Taxonomic Units (OTU) and aligned against SILVA 132 reference database. OTUs are presented in different taxonomic levels for each time-point. These data present valuable information on the microbial communities of table olives, a dynamic niche that affect the final product quality. The data presented are related to the research article “Insights into the evolution of Greek style table olives microbiome stored under modified atmosphere: biochemical implications on the product quality” [1].

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          Cutadapt removes adapter sequences from high-throughput sequencing reads

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            The SILVA ribosomal RNA gene database project: improved data processing and web-based tools

            SILVA (from Latin silva, forest, http://www.arb-silva.de) is a comprehensive web resource for up to date, quality-controlled databases of aligned ribosomal RNA (rRNA) gene sequences from the Bacteria, Archaea and Eukaryota domains and supplementary online services. The referred database release 111 (July 2012) contains 3 194 778 small subunit and 288 717 large subunit rRNA gene sequences. Since the initial description of the project, substantial new features have been introduced, including advanced quality control procedures, an improved rRNA gene aligner, online tools for probe and primer evaluation and optimized browsing, searching and downloading on the website. Furthermore, the extensively curated SILVA taxonomy and the new non-redundant SILVA datasets provide an ideal reference for high-throughput classification of data from next-generation sequencing approaches.
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              Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2

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                Author and article information

                Contributors
                Journal
                Data Brief
                Data Brief
                Data in Brief
                Elsevier
                2352-3409
                20 August 2021
                October 2021
                20 August 2021
                : 38
                : 107314
                Affiliations
                [a ]Institute of Applied Biosciences / CERTH, P.O. Box 60361, Thermi, Thessaloniki 57001, Greece
                [b ]Pelopac S.A., Block 38, NB1A Street, P.O. Box 1298, Thessaloniki Industrial Area, Sindos 57022, Greece
                [c ]Department of Food Science and Nutrition, University of the Aegean, Lemnos 81400, Greece
                Author notes
                [* ]Corresponding author at: Institute of Applied Biosciences / CERTH, P.O. Box 60361, Thermi, Thessaloniki 57001, Greece. argiriou@ 123456certh.gr
                Article
                S2352-3409(21)00598-9 107314
                10.1016/j.dib.2021.107314
                8397911
                65bb8a68-e9bd-4c9e-8504-520d570efaf4
                © 2021 The Authors. Published by Elsevier Inc.

                This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

                History
                : 5 August 2021
                : 18 August 2021
                Categories
                Data Article

                metagenomics,taxonomy,16s rrna,18s rrna,olives,modified atmosphere

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