A dual signal amplification-assisted DNAzyme biosensor for ultrasensitive detection of Argonaute 2 activity.

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Abstract

We develop a new DNAzyme biosensor for Argonaute 2 (Ago2) assay based on target-initiated rolling circle amplification in combination with an 8-17 DNAzyme-mediated dual signal amplification strategy. This biosensor can achieve ultrahigh sensitivity with a detection limit of 0.35 pM, and it can be further used for the screening of Ago2 inhibitors and the accurate measurement of endogenous Ago2 from HeLa cell extracts.

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Author and article information

Journal

Journal ID (iso-abbrev): Chem Commun (Camb)

Title: Chemical communications (Cambridge, England)

Publisher: Royal Society of Chemistry (RSC)

ISSN (Electronic): 1364-548X

ISSN (Print): 1359-7345

Publication date (Electronic): Dec 04 2018

Volume: 54

Issue: 97

Affiliations

[1 ] College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan 250014, China. cyzhang@sdnu.edu.cn.

Article

DOI: 10.1039/c8cc08553f

PubMed ID: 30451248

SO-VID: 653fe4d5-1ecf-415d-84ec-e2ac4a04b03c

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