This study investigated the knowledge and perception of artificial intelligence among library and information science professionals in Zambia by surveying 245 randomly selected participants. The results show that the library and information science professionals had a favourable and encouraging outlook with regard to artificial intelligence but were also concerned about artificial intelligence replacing librarians’ roles and the barriers to adopting artificial intelligence technologies in Zambian libraries. The article suggests that libraries should consider the study’s findings before implementing artificial intelligence, particularly concerning technology and facilities, librarians’ proficiency in artificial intelligence, and leadership positions in artificial intelligence initiatives. The research can be used as a resource by library boards and associations to develop policies for implementing artificial intelligence in academic libraries, and fills a research vacuum in developing nations like Zambia regarding the knowledge of university and college libraries, and their willingness to use artificial intelligence.