Pollinator scarcity drives the shift to delayed selfing in Himalayan mayapple Podophyllum hexandrum (Berberidaceae)

Abstract

American mayapple and Himalayan mayapple are a pair of sister species with disjunct distribution between eastern Asia and eastern North America, which the former was considered to be self-incompatible but the later to be self-compatible. We are interested in the diversification of breeding systems in the two species, particularly a usual mode of self pollination in Himalayan mayapple ( Podophyllum hexandrum) which is achieved by movement of the pistil as a previous study suggested. By contrast, our observations and flower manipulations show that delayed selfing was facilitated by petals closing and stamens moving simultaneously to contact the stigma, and bees were effective pollinators although they were few.

Abstract

Recent molecular phylogenetics have indicated that American mayapple (mainly self-incompatible, SI) and Himalayan mayapple, which was considered to be self-compatible (SC), are sister species with disjunct distribution between eastern Asia and eastern North America. We test a hypothesis that the persistence of this early spring flowering herb in the Himalayan region is attributable to the transition from SI to SC, the capacity for selfing in an unpredictable pollination environment. Pollinator observations were conducted in an alpine meadow with hundreds of Himalayan mayapple ( Podophyllum hexandrum Royle) individuals over 2 years. To examine autogamy, seed set under different pollination treatments was compared. To clarify whether automatic self-pollination is achieved by movement of the pistil as a previous study suggested, we measured incline angles of the pistil and observed flower movement during anthesis using video. Floral visitors to the nectarless flowers were very rare, but solitary bees and honeybees could be potential pollinators. Seed set of bagged flowers was not significantly different from that of open-pollinated, self- or cross-pollinated flowers. However, removal of petals or stamens lowered seed yield. The angles of inclination of pistils did not change during the process of pollination. Automatic self-pollination was facilitated by petals closing and stamens moving simultaneously to contact the stigma. Stigmatic pollen load increased little during the day time, in contrast to a sharp increase when the flowers closed during the night-time. These observations indicated that Himalayan mayapple was SC and delayed self-pollination was facilitated by the movement of petals rather than the pistil. Compared with SI American mayapple, no obvious inbreeding depression in SC Himalayan mayapple may contribute its existence in the uplifting zone. A scarcity of pollinators may have driven the shift to delayed selfing in P. hexandrum.