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      Antifungal potential of biosurfactants produced by strains of Bacillus mojavensis PS17 against pathogens of the genera Fusarium and Verticillium

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          Abstract

          Phytopathogenic fungi cause significant damage to agriculture, reducing or destroying the crop. There are several ways to control fungal diseases, such as the use of disease-resistant plant varieties, organic farming, and the use of fungicides. One of the methods of combating phytopathogens is the use of biological products, one of which is biosurfactants. Biosurfactants are surfactants produced in the course of life. They are able to reduce fungal growth and increase plant disease resistance, improve plant growth and yield, and act as adjuvants to improve wetting and solubility of fertilizers. In the course of this work, the fungicidal activity of biosurfactants formed by Bacillus mojavensis PS17 was assessed against pathogens of the genera Fusarium and Verticillium. It has been established that the biosurfactant is able to reduce the coverage area of Fusarium and Verticillium fungi in in vitro experiments by 39 and 43%. Thus, it can be concluded that the biosurfactant produced by Bacillus mojavensis PS17 has antifungal activity.

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          Diversity, pathogenicity, and management of verticillium species.

          The genus Verticillium encompasses phytopathogenic species that cause vascular wilts of plants. In this review, we focus on Verticillium dahliae, placing emphasis on the controversy surrounding the elevation of a long-spored variant as a new species, recent advances in the analysis of compatible and incompatible interactions, highlighted by the use of strains expressing fluorescent proteins, and the genetic diversity among Verticillium spp. A synthesis of the approaches to explore genetic diversity, gene flow, and the potential for cryptic recombination is provided. Control of Verticillium wilt has relied on a panoply of chemical and nonchemical strategies, but is beset with environmental or site-specific efficacy problems. Host resistance remains the most logical choice, but is unavailable in most crops. The genetic basis of resistance to Verticillium wilt is unknown in most crops, as are the subcellular signaling mechanisms associated with Ve-mediated, race-specific resistance. Increased understanding in each of these areas promises to facilitate management of Verticillium wilts across a broad range of crops.
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            Biosurfactants: Multifunctional Biomolecules of the 21st Century

            In the era of global industrialisation, the exploration of natural resources has served as a source of experimentation for science and advanced technologies, giving rise to the manufacturing of products with high aggregate value in the world market, such as biosurfactants. Biosurfactants are amphiphilic microbial molecules with hydrophilic and hydrophobic moieties that partition at liquid/liquid, liquid/gas or liquid/solid interfaces. Such characteristics allow these biomolecules to play a key role in emulsification, foam formation, detergency and dispersal, which are desirable qualities in different industries. Biosurfactant production is considered one of the key technologies for development in the 21st century. Besides exerting a strong positive impact on the main global problems, biosurfactant production has considerable importance to the implantation of sustainable industrial processes, such as the use of renewable resources and “green” products. Biodegradability and low toxicity have led to the intensification of scientific studies on a wide range of industrial applications for biosurfactants in the field of bioremediation as well as the petroleum, food processing, health, chemical, agricultural and cosmetic industries. In this paper, we offer an extensive review regarding knowledge accumulated over the years and advances achieved in the incorporation of biomolecules in different industries.
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              Plant defense stimulation by natural isolates of bacillus depends on efficient surfactin production.

              Some plant-associated Bacillus strains produce induced systemic resistance (ISR) in the host, which contributes to their protective effect against phytopathogens. Little is known about the variety of elicitors responsible for ISR that are produced by Bacillus strains. Working with a particular strain, we have previously identified the surfactin lipopeptide as a main compound stimulating plant immune-related responses. However, with the perspective of developing Bacillus strains as biocontrol agents, it is important to establish whether a central role of surfactin is generally true for isolates belonging to the B. subtilis/amyloliquefaciens complex. To that end, we set up a comparative study involving a range of natural strains. Their secretomes were first tested for triggering early defense events in cultured tobacco cells. Six isolates with contrasting activities were further evaluated for ISR in plants, based both on macroscopic disease reduction and on stimulation of the oxylipin pathway as defense mechanism. A strong correlation was found between defense-inducing activity and the amount of surfactin produced by the isolates. These results support the idea of a widespread role for surfactin as a nonvolatile elicitor formed by B. subtilis/amyloliquefaciens, and screening for strong surfactin producers among strains naturally secreting multiple antibiotics could be an efficient approach to select good candidates as biopesticides.
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                Author and article information

                Journal
                BIO Web of Conferences
                BIO Web Conf.
                EDP Sciences
                2117-4458
                2023
                November 07 2023
                2023
                : 71
                : 01102
                Article
                10.1051/bioconf/20237101102
                6213243c-ceef-4232-87e7-c0a8d8e0f2b2
                © 2023

                https://creativecommons.org/licenses/by/4.0/

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