Chlorophyll determination in intact tissues using n,n-dimethylformamide. – ScienceOpen
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      Chlorophyll determination in intact tissues using n,n-dimethylformamide.

      1 ,
      Plant physiology
      American Society of Plant Biologists (ASPB)

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          Abstract

          Photosynthetic pigments from etiolated cucumber (Cucumis sativus var. Beit Alpha improved, Hazera Co., Gedera) cotyledons were extracted by direct immersion of the intact cotyledons into the solvent N,N-dimethylformamide (DMF). The solvent is especially efficient when pigment concentration is low; time and tools are saved and the loss of pigment that usually occurs in more complicated extraction procedures is prevented. The specific absorption coefficient of chlorophyll a in DMF was also determined.

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          Author and article information

          Journal
          Plant Physiol
          Plant physiology
          American Society of Plant Biologists (ASPB)
          0032-0889
          0032-0889
          Mar 1980
          : 65
          : 3
          Affiliations
          [1 ] Department of Botany, The George S. Wise Faculty for Life Sciences, Tel Aviv University, Ramat Aviv, Israel.
          Article
          10.1104/pp.65.3.478
          440358
          16661217
          615a4269-4624-4c68-bafd-30397a11c28b
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