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      Identification of metabolic markers in coronary artery disease using an untargeted LC-MS based metabolomic approach.

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          Abstract

          Coronary artery disease (CAD), a complex metabolic disorder, is one of the largest causes of death worldwide. Both environmental and genetic factors contribute to the etiology of this metabolic disease. The gene-environment interaction could lead to modulation of various metabolic pathways resulting in altered levels of various metabolites. Thus, identifying metabolites could aid in deciphering pathways that could be involved in the pathophysiology of the disease. With the advent of high resolution mass spectrometry based methodologies, it is now possible to screen thousands of metabolites in a single snapshot thus, allowing the identification of potential disease metabolite markers. In this work, using an untargeted metabolomic approach, we attempted to identify metabolites that have altered levels in CAD patients. Using reverse phase and HILIC based chromatography followed by mass spectrometry we identified a total of 32 metabolites (2 fold; p<0.05) in plasma whose levels were significantly altered in CAD samples. Further, we have validated the discriminative ability of these metabolites in an independent set of CAD and control samples using multivariate PLS-DA analysis. Interestingly, Lyso PC (18:0), Cortisol, Lyso PC (P-17:0), and glycerophosphocholine were among the top discriminators for CAD which implies involvement of phosphatidylcholine pathway in the pathogenesis of atherosclerosis.

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          Author and article information

          Journal
          J Proteomics
          Journal of proteomics
          1876-7737
          1874-3919
          Sep 8 2015
          : 127
          : Pt A
          Affiliations
          [1 ] Genomics and Molecular Medicine Unit, CSIR-Institute of Genomics and Integrative Biology, SukhdevVihar, Mathura Road, New Delhi 110020, India; Academy of Scientific & Innovative Research (AcSIR), CSIR-IGIB Campus, New Delhi, India.
          [2 ] Genomics and Molecular Medicine Unit, CSIR-Institute of Genomics and Integrative Biology, SukhdevVihar, Mathura Road, New Delhi 110020, India.
          [3 ] Dept. of Cardiology, All India Institute of Medical Sciences, New Delhi, India.
          [4 ] Genomics and Molecular Medicine Unit, CSIR-Institute of Genomics and Integrative Biology, SukhdevVihar, Mathura Road, New Delhi 110020, India; Academy of Scientific & Innovative Research (AcSIR), CSIR-IGIB Campus, New Delhi, India. Electronic address: shantanus@igib.res.in.
          Article
          S1874-3919(15)00095-0
          10.1016/j.jprot.2015.03.011
          25790721
          61381ce7-b190-4e43-ad75-c5497399c8ce
          Copyright © 2015 Elsevier B.V. All rights reserved.
          History

          Coronary artery disease,LC-MS,PLS-DA,Plasma,Untargeted-metabolomics

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