CBMT-38. ASSOCIATION OF miR-146aC>G, miR-149C>T, miR-196a2C>T, AND miR-499A>G POLYMORPHISMS WITH BRAIN TUMORS

Abstract

MicroRNAs (miRNAs) are short, noncoding RNAs that are implicated in tumorigenesis, functioning as both tumor suppressors and oncogenes. However, the clinical significance of miRNA expression profiles for brain tumors remains unclear. Therefore, we designed a study to investigate the association between microRNA genetic variants and brain tumor risk. We recruited 362 participants; 179 for the healthy subjects and 183 who were brain tumor patients confirmed as having gliomas, meningiomas, or schwannomas. This study investigated the single nucleotide polymorphisms miR-146aC>G, miR-149T>C, miR-196a2T>C, and miR-499A>G by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). We found that the dominant miR-149 and CC genotypes were significantly more frequent in patients with glioma. The odds ratios for the C-C-C-G, C-T-C-G, and G-C-T-G haplotypes ( miR-146aC>G- miR-149T>C- miR-196a2T>C- miR-499A>G) were significantly increased in glioma, as was the odds ratio for the GCT haplotype of miR-146aC>G, miR-149T>C, and miR-196a2T>C and for the CCG haplotype of miR-149T>C, miR-196a2T>C, and miR-499A>G. In meningioma, the odds ratios were increased in the GTCG haplotype of miR-146aC>G, miR-149T>C, miR-196a2T>C, and miR-499A>G. The odds ratios were also increased in the GCG haplotype of miR-146aC>G, miR-196a2T>C, and miR-499A>G and CCG haplotype of miR-149T>C, miR-1962T>C, and miR-499A>G. The odds ratios for schwannoma were increased in the GCTG haplotype of miR-146aC>G, miR-149T>C, miR-196a2T>C, and miR-499A>G and the CCG haplotype of miR-149T>C, miR-196a2T>C, and miR-499A>G. In conclusion, our results suggested that the miR-149 polymorphism might be involved in the development of gliomas, and the CCG haplotype of miR-149T>C, miR-196a2T>C, and miR-499A>G showed increased odds ratios for all types of brain tumors in Koreans.