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      The millennial dynamics of malaria in the mediterranean basin: documenting Plasmodium spp. on the medieval island of Corsica

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          Abstract

          Introduction

          The lack of well-preserved material upon which to base the paleo-microbiological detection of Plasmodium parasites has prevented extensive documentation of past outbreaks of malaria in Europe. By trapping intact erythrocytes at the time of death, dental pulp has been shown to be a suitable tissue for documenting ancient intraerythrocytic pathogens such as Plasmodium parasites.

          Methods

          Total DNA and proteins extracted from 23 dental pulp specimens collected from individuals exhumed from the 9th to 13th century archaeological site in Mariana, Corsica, were analyzed using open-mind paleo-auto-immunohistochemistry and direct metagenomics, Plasmodium-targeting immunochromatography assays. All experiments incorporated appropriate negative controls.

          Results

          Paleo-auto-immunohistochemistry revealed the presence of parasites Plasmodium spp. in the dental pulp of nine teeth. A further immunochromatography assay identified the presence of at least one Plasmodium antigen in nine individuals. The nine teeth, for which the PfHRP-2 antigen specific of P. falciparum was detected, were also positive using paleo-autoimmunohistochemistry and metagenomics.

          Conclusion

          Dental pulp erythrocytes proved to be suitable for the direct paleomicrobiology documentation of malaria in nine individuals buried in medieval Corsica, in agreement with historical data. This provides additional information on the millennial dynamics of Plasmodium spp. in the Mediterranean basin.

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          Most cited references49

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          Improved metagenomic analysis with Kraken 2

          Although Kraken’s k-mer-based approach provides a fast taxonomic classification of metagenomic sequence data, its large memory requirements can be limiting for some applications. Kraken 2 improves upon Kraken 1 by reducing memory usage by 85%, allowing greater amounts of reference genomic data to be used, while maintaining high accuracy and increasing speed fivefold. Kraken 2 also introduces a translated search mode, providing increased sensitivity in viral metagenomics analysis.
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            A method for visual determination of sex, using the human hip bone.

            J Brůzek (2002)
            A new visual method for the determination of sex using the human hip bone (os coxae) is proposed, based on a revision of several previous approaches which scored isolated characters of this bone. The efficacy of the methodology is tested on a sample of 402 adults of known sex and age of French and Portuguese origins. With the simultaneous use of five characters of the hip bone, it is possible to provide a correct sexual diagnosis in 95% of all cases, with an error of 2% and an inability to identify sex in only 3%. The advantage of this new method is a reduction in observer subjectivity, since the evaluation procedure cannot involve any anticipation of the result. In addition, this method of sex determination increases the probability of a correct diagnosis with isolated fragments of the hip bone, provided that a combination of elements of one character is found to be typically male or female. Copyright 2002 Wiley-Liss, Inc.
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              World malaria report 2022.

              (2022)
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                Author and article information

                Contributors
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                URI : http://loop.frontiersin.org/people/1175625/overviewRole: Role: Role: Role: Role: Role: Role: Role: Role: Role: Role:
                Journal
                Front Med (Lausanne)
                Front Med (Lausanne)
                Front. Med.
                Frontiers in Medicine
                Frontiers Media S.A.
                2296-858X
                08 December 2023
                2023
                : 10
                : 1265964
                Affiliations
                [1] 1IHU Méditerranée Infection , Marseille, France
                [2] 2Aix-Marseille Univ, Institut de recherche pour le développement , Microbes, Evolution, Phylogénie et Infection, IHU Méditerranée Infection , Marseille, France
                [3] 3Aix-Marseille Univ, Centre national de la recherche scientifique, LA3M , Aix-en-Provence, France
                [4] 4Aix-Marseille Université, Centre national de la recherche scientifique, Établissement français du sang, Anthropologie bio-culturelle, droit, éthique et santé , Marseille, France
                [5] 5Unité Parasitologie et Entomologie, Département Microbiologie et Maladies Infectieuses, Institut de Recherche Biomédicale des Armées , Marseille, France
                [6] 6Aix-Marseille Univ, Institut de recherche pour le développement, Service de Santé des Armées, Assistance publique - Hôpitaux de Marseille, VITROME , Marseille, France
                [7] 7Centre National de Référence du Paludisme , Marseille, France
                Author notes

                Edited by: Sahar Saleem, Cairo University, Egypt

                Reviewed by: Gerhard Wunderlich, University of São Paulo, Brazil; Mircea Ioan Popa, Carol Davila University of Medicine and Pharmacy, Romania

                *Correspondence: Bruno Pradines, bruno.pradines@ 123456gmail.com
                Article
                10.3389/fmed.2023.1265964
                10739463
                38143446
                58eb8f32-d885-4563-b9a7-4db341d1d880
                Copyright © 2023 Boualam, Corbara, Aboudharam, Istria, Signoli, Costedoat, Drancourt and Pradines.

                This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

                History
                : 24 July 2023
                : 13 November 2023
                Page count
                Figures: 7, Tables: 1, Equations: 0, References: 50, Pages: 11, Words: 7390
                Funding
                Funded by: Délégation Générale pour l'Armement, doi 10.13039/501100005902;
                The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This study was supported by the Délégation Générale des Armées (grant NBC-2-B-2120).
                Categories
                Medicine
                Original Research
                Custom metadata
                Pathology

                plasmodium,plasmodium falciparum,plasmodium ovale,ancient malaria,corsica,paleo-auto-immunohistochemistry,metagenomic,immunochromatography

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