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      • Record: found
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      MicroRNA-1247 inhibits cell proliferation by directly targeting ZNF346 in childhood neuroblastoma

      research-article
      Author(s): 1 , 2 , 1 ,
      Publication date (Electronic):
      Journal: Biological Research
      Publisher: BioMed Central
      Keywords: Neuroblastoma, miR-1247, Proliferation, Apoptosis, ZNF346

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          Abstract

          Background

          Neuroblastoma (NB) represents the most common extracranial solid tumor in children. Accumulating evidence shows that microRNAs (miRs) play an important role in the carcinogenesis of NB. Here, we investigated the biological function of miR-1247 in NB in vitro.

          Methods/results

          We found miR-1247 was downregulated in NB tissues and cells using quantitative PCR analysis. Gain- and loss-of-function studies demonstrated that miR-1247 significantly suppressed cell proliferation and induced cell cycle G0/G1 phase arrest and cell apoptosis of NB cells in vitro by using MTT, colony formation assay and Flow cytometry analysis. Luciferase assay suggested ZNF346 was the target of miR-1247 and its expression could be downregulated by miR-1247 overexpression using Western blotting. Furthermore, downregulation of ZNF346 by siRNA performed similar effects with overexpression of miR-1247 in NB cells.

          Conclusions

          Our findings suggested miR-1247 directly targeted to repress ZNF346 expression, thus suppressing the progression of NB, which might be a novel therapeutic target against NB.

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          Most cited references30

          • Record: found
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          Combinatorial microRNA target predictions.

          Azra Krek, Dominic Grün, Matthew Poy … (2005)
          MicroRNAs are small noncoding RNAs that recognize and bind to partially complementary sites in the 3' untranslated regions of target genes in animals and, by unknown mechanisms, regulate protein production of the target transcript. Different combinations of microRNAs are expressed in different cell types and may coordinately regulate cell-specific target genes. Here, we present PicTar, a computational method for identifying common targets of microRNAs. Statistical tests using genome-wide alignments of eight vertebrate genomes, PicTar's ability to specifically recover published microRNA targets, and experimental validation of seven predicted targets suggest that PicTar has an excellent success rate in predicting targets for single microRNAs and for combinations of microRNAs. We find that vertebrate microRNAs target, on average, roughly 200 transcripts each. Furthermore, our results suggest widespread coordinate control executed by microRNAs. In particular, we experimentally validate common regulation of Mtpn by miR-375, miR-124 and let-7b and thus provide evidence for coordinate microRNA control in mammals.
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            The genetic landscape of high-risk neuroblastoma

            Trevor J Pugh, Olena Morozova, Edward F. Attiyeh … (2013)
            Neuroblastoma is a malignancy of the developing sympathetic nervous system that often presents with widespread metastatic disease, resulting in survival rates of less than 50% 1 . To determine the spectrum of somatic mutation in high-risk neuroblastoma, we studied 240 cases using a combination of whole exome, genome and transcriptome sequencing as part of the Therapeutically Applicable Research to Generate Effective Treatments (TARGET) initiative. Here we report a low median exonic mutation frequency of 0.60 per megabase (0.48 non-silent), and remarkably few recurrently mutated genes in these tumors. Genes with significant somatic mutation frequencies included ALK (9.2% of cases), PTPN11 (2.9%), ATRX (2.5%, an additional 7.1% had focal deletions), MYCN (1.7%, a recurrent p.Pro44Leu alteration), and NRAS (0.83%). Rare, potentially pathogenic germline variants were significantly enriched in ALK, CHEK2, PINK1, and BARD1. The relative paucity of recurrent somatic mutations in neuroblastoma challenges current therapeutic strategies reliant upon frequently altered oncogenic drivers.
            Article 0 comments Cited 243 times – based on 0 reviews     Review now
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              Identification of tissue-specific microRNAs from mouse.

              Mariana Lagos-Quintana, Reinhard Rauhut, Abdullah Yalcin … (2002)
              MicroRNAs (miRNAs) are a new class of noncoding RNAs, which are encoded as short inverted repeats in the genomes of invertebrates and vertebrates. It is believed that miRNAs are modulators of target mRNA translation and stability, although most target mRNAs remain to be identified. Here we describe the identification of 34 novel miRNAs by tissue-specific cloning of approximately 21-nucleotide RNAs from mouse. Almost all identified miRNAs are conserved in the human genome and are also frequently found in nonmammalian vertebrate genomes, such as pufferfish. In heart, liver, or brain, it is found that a single, tissue-specifically expressed miRNA dominates the population of expressed miRNAs and suggests a role for these miRNAs in tissue specification or cell lineage decisions. Finally, a miRNA was identified that appears to be the fruitfly and mammalian ortholog of C. elegans lin-4 stRNA.
              Article 0 comments Cited 238 times – based on 0 reviews     Review now
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                Author and article information

                Contributors
                Tingting Wu: wutingting_02@126.com
                Yun Lin: lin_Y76@126.com
                Zhongguo Xie: +86-0716-8211787 , xieZ_guo@163.com
                Journal
                Journal ID (nlm-ta): Biol Res
                Journal ID (iso-abbrev): Biol. Res
                Title: Biological Research
                Publisher: BioMed Central (London )
                ISSN (Print): 0716-9760
                ISSN (Electronic): 0717-6287
                Publication date (Electronic): 24 May 2018
                Publication date PMC-release: 24 May 2018
                Publication date Collection: 2018
                Volume: 51
                Electronic Location Identifier: 13
                Affiliations
                [1 ]GRID grid.459509.4, Department of Neonatology, , The First People’s Hospital of Jingzhou, ; No. 8 Hangkong Road, Shashi District, Jingzhou, 434000 Hubei China
                [2 ]ISNI 0000 0004 1808 0950, GRID grid.410646.1, Department of Editor, , Sichuan Academy of Medical Sciences and Sichuan Provincial People’s Hospital, ; Sichuan, China
                Article
                Publisher ID: 162
                DOI: 10.1186/s40659-018-0162-y
                PMC ID: 5966945
                PubMed ID: 29793538
                SO-VID: 58bb4372-5062-44c1-bd4c-d6da9952f300
                Copyright © © The Author(s) 2018
                License:

                Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License ( http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

                History
                Date received : 9 November 2017
                Date accepted : 21 May 2018
                Categories
                Subject: Research Article
                Custom metadata
                issue-copyright-statement © The Author(s) 2018

                Keywords: neuroblastoma,mir-1247,proliferation,apoptosis,znf346
                Data availability:
                Keywords: neuroblastoma, mir-1247, proliferation, apoptosis, znf346

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