11
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Anti-tumor effects of paeonol in melanoma by up-regulating miR-143 and suppressing its target WNT5B

      ,
      STEMedicine
      Innovation Publishing House Pte. Ltd.

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Previous studies have shown the potent anti-tumor effect of paeonol, a natural compound extracted from traditional Chinese herb medicine. However, the therapeutic effect and underlying mechanisms of paeonol in melanoma are largely unknown. Two melanoma cell lines (M14 and A375) were cultured in the presence of paeonol. Cell proliferation was determined by colony formation and cell-counting kit 8 assays. Real-time quantitative reverse transcription polymerase chain reaction and Western blot were performed to detect mRNA and protein expression, respectively. Paeonol treatment significantly inhibited the colony formation and proliferation of melanoma cells by enhancing the expression of the well-characterized tumor suppressor microRNA (miR)-143. Block of miR-143 diminished the inhibitory effects of paeonol on cell growth. miR-143 directly bound to Wnt family member 5B (WNT5B) gene. Paeonol therefore down-regulated WNT5B expression through miR-143. Moreover, paeonol suppressed melanoma growth in vivo. Our study reveals that paeonol effectively suppresses melanoma cell growth both in vitro and in vivo through miR-143/WNT5B axis, which shows potential for the treatment of melanoma.

          Related collections

          Author and article information

          Journal
          STEMedicine
          STEMedicine
          Innovation Publishing House Pte. Ltd.
          2705-1188
          January 07 2023
          January 20 2023
          : 4
          : 1
          : e161
          Article
          10.37175/stemedicine.v4.i1.161
          55d802f6-137f-4148-9610-664e37caf03f
          © 2023

          https://creativecommons.org/licenses/by/4.0

          History

          Comments

          Comment on this article