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      Nutrients, minerals, antioxidant pigments and phytochemicals, and antioxidant capacity of the leaves of stem amaranth

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      1 , , 2 , 3
      Scientific Reports
      Nature Publishing Group UK
      Biochemistry, Plant sciences

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          Abstract

          We evaluated 17 genotypes of stem amaranth ( Amaranthus lividus) in terms of dietary fiber, moisture, carbohydrates, fat, ash, gross energy, protein, minerals, phytopigments, total antioxidant capacity (TAC), vitamins, total flavonoids (TFC), total polyphenols (TPC) and their variations. Stem amaranth leaves have abundant dietary fiber, moisture, carbohydrates, and protein. We found significant amount of potassium, calcium, magnesium (9.61, 24.40, and 29.77 mg g −1 DW), iron, manganese, copper, zinc, (1131.98, 269.89, 25.03, and 1006.53 µg g −1 DW), phytopigments such as chlorophyll a, chlorophyll ab chlorophyll b, (27.76, 42.06, and 14.30 mg 100 g −1 FW), betalain, betaxanthin, betacyanin (62.92, 31.81, 31.12 µg 100 g −1 FW), total carotenoids, beta-carotene (1675.38, 1289.26 µg g −1 FW), vitamin C (1355.46 µg g −1 FW), TPC, TFC (228.63 GAE and 157.42 RE µg g −1 DW), and TAC (DPPH, ABTS +) (26.61, 51.73 TEAC µg g −1 DW) in the leaves of stem amaranth. Genotypes exhibited a wide range of variations. Three genotypes DS40, DS30, and DS26 could be used as an antioxidant profile enriched stem amaranth. Phenolics, phytopigments, flavonoids, and vitamins of stem amaranth leaves exhibited strong antioxidant activity. Stem amaranth could be a potential source of dietary fiber, moisture, carbohydrates, protein, minerals, phenolics, phytopigments, flavonoids, and vitamins in our daily diet for attaining nutritional and antioxidant sufficiency.

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          Drought stress enhances nutritional and bioactive compounds, phenolic acids and antioxidant capacity of Amaranthus leafy vegetable

          Background Bioactive compounds, vitamins, phenolic acids, flavonoids of A. tricolor are the sources of natural antioxidant that had a great importance for the food industry as these detoxify ROS in the human body. These natural antioxidants protect human from many diseases such as cancer, arthritis, emphysema, retinopathy, neuro-degenerative cardiovascular diseases, atherosclerosis and cataracts. Moreover, previous literature has shown that drought stress elevated bioactive compounds, vitamins, phenolics, flavonoids and antioxidant activity in many leafy vegetables. Hence, we study the nutritional and bioactive compounds, phenolic acids, flavonoids and antioxidant capacity of amaranth under drought stress for evaluation of the significant contribution of these compounds in the human diet. Results The genotype VA3 was assessed at four drought stress levels that significantly affected nutritional and bioactive compounds, phenolic acids, flavonoids and antioxidant capacity. Protein, ash, energy, dietary fiber, Ca, K, Cu, S, Mg, Mn, Mo, Na, B content, total carotenoids, TFC, vitamin C, TPC, TAC (DPPH), betacarotene, TAC (ABTS+), sixteen phenolic acids and flavonoids were remarkably increased with the severity of drought stress. At moderate and severe drought stress conditions, the increments of all these components were more preponderant. Trans-cinnamic acid was newly identified phenolic acid in A. tricolor. Salicylic acid, vanilic acid, gallic acid, chlorogenic acid, Trans-cinnamic acid, rutin, isoquercetin, m-coumaric acid and p-hydroxybenzoic acid were the most abundant phenolic compounds in this genotype. Conclusions In A. tricolor, drought stress enhanced the quantitative and qualitative improvement of nutritional and bioactive compounds, phenolic acids, flavonoids and antioxidants. Hence, farmers of semi-arid and dry areas of the world could be able to grow amaranth as a substitute crop.
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            Catalase, superoxide dismutase and ascorbate-glutathione cycle enzymes confer drought tolerance of Amaranthus tricolor

            The study was performed to explore physiological, non-enzymatic and enzymatic detoxification pathways of reactive oxygen species (ROS) in tolerance of Amaranthus tricolor under drought stress. The tolerant genotype VA13 exhibited lower reduction in growth, photosynthetic pigments, relative water content (RWC) and negligible increment in electrolyte leakage (EL), lower increment in proline, guaiacol peroxidase (GPOX) activity compared to sensitive genotype VA15. This genotype also had higher catalase (CAT), superoxide dismutase (SOD), remarkable and dramatic increment in ascorbate-glutathione content, ascorbate-glutathione redox and ascorbate-glutathione cycle enzymes activity compared to sensitive genotype VA15. The negligible increment of ascorbate-glutathione content, ascorbate-glutathione redox and ascorbate-glutathione cycle enzymes activities and dramatic increment in malondialdehyde (MDA), hydrogen peroxide (H2O2) and EL were observed in the sensitive genotype VA15. SOD contributed superoxide radical dismutation and CAT contributed H2O2 detoxification in both sensitive and tolerant varieties, however, these had a great contribution in the tolerant variety. Conversely, proline and GPOX accumulation were higher in the sensitive variety compared to the tolerant variety. Increase in ascorbate-glutathione cycle enzymes activities, CAT, ascorbate-glutathione content, SOD, and ascorbate-glutathione redox clearly evident that CAT, ascorbate-glutathione cycle and SOD played a significant activity in ROS detoxification of tolerant A. tricolor variety.
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              Flavonoids and other phenolic compounds in Andean indigenous grains: Quinoa (Chenopodium quinoa), kañiwa (Chenopodium pallidicaule) and kiwicha (Amaranthus caudatus)

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                Author and article information

                Contributors
                umakanta@bsmrau.edu.bd
                Journal
                Sci Rep
                Sci Rep
                Scientific Reports
                Nature Publishing Group UK (London )
                2045-2322
                3 March 2020
                3 March 2020
                2020
                : 10
                : 3892
                Affiliations
                [1 ]GRID grid.443108.a, Department of Genetics and Plant Breeding, Faculty of Agriculture, , Bangabandhu Sheikh Mujibur Rahman Agricultural University, ; Gazipur, 1706 Bangladesh
                [2 ]ISNI 0000 0004 0370 4927, GRID grid.256342.4, Laboratory of Field Science, Faculty of Applied Biological Sciences, , Gifu University, ; Yanagido 1-1 Gifu, Japan
                [3 ]GRID grid.473322.3, Soil, Crop and Natural Resource Management, , Sierra Leone Agricultural Research Institute, ; Tower Hill Freetown Sierra Leone, PMB 1313 Sierra Leone
                Article
                60252
                10.1038/s41598-020-60252-7
                7054523
                32127553
                5586eab3-90a0-4157-a9c4-5a6dad9db01f
                © The Author(s) 2020

                Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

                History
                : 5 August 2019
                : 7 February 2020
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                biochemistry,plant sciences
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