Novel Hypocholesterolemic Peptides Derived from Silver Carp Muscle: The Modulatory Effects on Enterohepatic Cholesterol Metabolism In Vitro and In Vivo.

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Abstract

This research aimed to investigate the effect of silver carp hydrolysates (SCHs) on hypercholesterolemia and enterohepatic cholesterol metabolism. Results showed that in vitro gastrointestinal digestion products of Alcalase-SCH (GID-Alcalase) exhibited the highest inhibitory activity of cholesterol absorption mainly through downregulating the expression of essential genes related to cholesterol transport in a Caco-2 monolayer. After being absorbed by the Caco-2 monolayer, GID-Alcalase increased the low-density lipoprotein (LDL) uptake in HepG2 cells by enhancing the protein level of the LDL receptor (LDLR). The in vivo experiment showed that long-term intervention of Alcalase-SCH ameliorated hypercholesterolemia in ApoE-/- mice fed with a Western diet (WD). After transepithelial transport, four novel peptides (TKY, LIL, FPK, and IAIM) were identified, and these peptides possessed dual hypocholesterolemic functions including inhibition of cholesterol absorption and promotion of peripheric LDL uptake. Our results indicated for the first time the potential of SCHs as functional food ingredients for the management of hypercholesterolemia.

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Author and article information

Journal

Journal ID (iso-abbrev): J Agric Food Chem

Title: Journal of agricultural and food chemistry

Publisher: American Chemical Society (ACS)

ISSN (Electronic): 1520-5118

ISSN (Print): 0021-8561

Publication date (Electronic): Apr 12 2023

Volume: 71

Issue: 14

Affiliations

[1 ] Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.

[2 ] Department of Animal and Food Sciences, University of Kentucky, Lexington, Kentucky 40546, United States.

[3 ] National Research and Development Center for Freshwater Fish Processing, Jiangxi Normal University, Nanchang, Jiangxi 330022, China.

Article

DOI: 10.1021/acs.jafc.2c09092

PubMed ID: 36997503

SO-VID: 54de8fef-64fb-4360-8418-29aaa50eba58

History

Keywords: hypercholesterolemia mice,peptide sequences,silver carp muscle hydrolysate,transepithelial transport,LDL uptake,cholesterol absorption

Data availability:

Keywords: hypercholesterolemia mice, peptide sequences, silver carp muscle hydrolysate, transepithelial transport, LDL uptake, cholesterol absorption

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