Reduction in Oviposition of Poultry Red Mite ( Dermanyssus gallinae ) in Hens Vaccinated with Recombinant Akirin

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Abstract

The poultry red mite (PRM), Dermanyssus gallinae, is a hematophagous ectoparasite of birds with worldwide distribution that causes economic losses in the egg-production sector of the poultry industry. Traditional control methods, mainly based on acaricides, have been only partially successful, and new vaccine-based interventions are required for the control of PRM. Vaccination with insect Akirin (AKR) and its homolog in ticks, Subolesin (SUB), have shown protective efficacy for the control of ectoparasite infestations and pathogen infection/transmission. The aim of this study was the identification of the akr gene from D. gallinae ( Deg-akr), the production of the recombinant Deg-AKR protein, and evaluation of its efficacy as a vaccine candidate for the control of PRM. The anti-Deg-AKR serum IgY antibodies in hen sera and egg yolk were higher in vaccinated than control animals throughout the experiment. The results demonstrated the efficacy of the vaccination with Deg-AKR for the control of PRM by reducing mite oviposition by 42% following feeding on vaccinated hens. A negative correlation between the levels of serum anti-Deg-AKR IgY and mite oviposition was obtained. These results support Deg-AKR as a candidate protective antigen for the control of PRM population growth.

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Tick vaccines: current status and future directions.

José Burón de la Fuente, Marinela Contreras (2015)

Ticks and tick-borne diseases are a growing problem affecting human and animal health worldwide. Traditional control methods, based primarily on chemical acaricides, have proven not to be sustainable because of the selection of acaricide-resistant ticks. Tick vaccines appear to be a promising and effective alternative for control of tick infestations and pathogen transmission. The purpose of this review is to summarize previous tick vaccine development and performance and formulate critical issues and recommendations for future directions for the development of improved and effective tick vaccines. The development of effective screening platforms and algorithms using omics approaches focused on relevant biological processes will allow the discovery of new tick-protective antigens. Future vaccines will likely combine tick antigens with different protective mechanisms alone or pathogen-derived antigens. The application of tick vaccines as a part of integrated control strategies will ultimately result in the control of tick-borne diseases.

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The tick protective antigen, 4D8, is a conserved protein involved in modulation of tick blood ingestion and reproduction.

José de la Fuente, Consuelo Almazán, Uriel Blas-Machado … (2006)

The gene that encodes the tick protective antigen, 4D8, was cloned from 10 species belonging to 6 genera, and the nucleotide and amino acid sequences were analyzed. 4D8 nucleotide and protein sequences were conserved among these tick species with identity/similarity between 65-98 and 60-98%, respectively. The function of 4D8 was characterized by RNA interference (RNAi) in five tick species. After the ticks were allowed to feed, degeneration of gut, salivary glands and reproductive tissues was observed, and tick survival, weight and oviposition were significantly reduced. 4D8 RNAi effected >90% reduction in oviposition in all tick species tested. Because of the critical role that 4D8 plays during tick feeding and oviposition, which ultimately results in the reduction of tick progeny, we proposed the generic name "subolesin" (Latin, suboles: offspring, progeny) for tick 4D8 proteins and subA for the subolesin-encoding gene.

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Vaccination with proteins involved in tick-pathogen interactions reduces vector infestations and pathogen infection.

Octavio Merino, Sandra Antunes, Juan Luis Mosqueda … (2013)

Tick-borne pathogens cause diseases that greatly impact animal health and production worldwide. The ultimate goal of tick vaccines is to protect against tick-borne diseases through the control of vector infestations and reducing pathogen infection and transmission. Tick genetic traits are involved in vector-pathogen interactions and some of these molecules such as Subolesin (SUB) have been shown to protect against vector infestations and pathogen infection. Based on these premises, herein we characterized the efficacy of cattle vaccination with tick proteins involved in vector-pathogen interactions, TROSPA, SILK, and Q38 for the control of cattle tick, Rhipicephalus (Boophilus) microplus infestations and infection with Anaplasma marginale and Babesia bigemina. SUB and adjuvant/saline placebo were used as positive and negative controls, respectively. The results showed that vaccination with Q38, SILK and SUB reduced tick infestations and oviposition with vaccine efficacies of 75% (Q38), 62% (SILK) and 60% (SUB) with respect to ticks fed on placebo control cattle. Vaccination with TROSPA did not have a significant effect on any of the tick parameters analyzed. The results also showed that vaccination with Q38, TROSPA and SUB reduced B. bigemina DNA levels in ticks while vaccination with SILK and SUB resulted in lower A. marginale DNA levels when compared to ticks fed on placebo control cattle. The positive correlation between antigen-specific antibody titers and reduction of tick infestations and pathogen infection strongly suggested that the effect of the vaccine was the result of the antibody response in vaccinated cattle. Vaccination and co-infection with A. marginale and B. bigemina also affected the expression of genes encoding for vaccine antigens in ticks fed on cattle. These results showed that vaccines using tick proteins involved in vector-pathogen interactions could be used for the dual control of tick infestations and pathogen infection. Copyright © 2013 Elsevier Ltd. All rights reserved.

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Journal

Journal ID (nlm-ta): Vaccines (Basel)

Journal ID (iso-abbrev): Vaccines (Basel)

Journal ID (publisher-id): vaccines

Title: Vaccines

Publisher: MDPI

ISSN (Electronic): 2076-393X

Publication date (Electronic): 19 September 2019

Publication date Collection: September 2019

Volume: 7

Issue: 3

Electronic Location Identifier: 121

Affiliations

[1 ]SaBio, Instituto de Investigación en Recursos Cinegéticos IREC (CSIC-UCLM-JCCM), Ronda de Toledo 12, 13071 Ciudad Real, Spain; josefranvet@ 123456gmail.com (J.F.L.-B.); marinelacr@ 123456hotmail.com (M.C.); marta.sanchez@ 123456uclm.es (M.S.-S.); ursula.hofle@ 123456uclm.es (U.H.); margaritam.villar@ 123456uclm.es (M.V.)

[2 ]Sabiotec, Ed. Polivalente UCLM, Camino de Moledores s/n, 13005 Ciudad Real, Spain

[3 ]Moredun Research Institute, Pentlands Science Park, Bush Loan, Edinburgh, Midlothian EH26 0PZ, UK; kathryn.bartley@ 123456moredun.ac.uk (K.B.); dan.price@ 123456moredun.ac.uk (D.R.G.P.); francesca.nunn@ 123456moredun.ac.uk (F.N.); alasdair.nisbet@ 123456moredun.ac.uk (A.J.N.)

[4 ]Department of Applied Physics, Faculty of Science, University of Castilla La Mancha, Avda. Camilo José Cela 10, 13071 Ciudad Real, Spain; eduardo.prado@ 123456uclm.es

[5 ]Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078, USA

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[* ]Correspondence: jose_delafuente@ 123456yahoo.com

Author information

Jose Francisco Lima-Barbero https://orcid.org/0000-0002-2694-0215

Eduardo Prado https://orcid.org/0000-0001-8279-6310

Ursula Höfle https://orcid.org/0000-0002-6868-079X

Margarita Villar https://orcid.org/0000-0003-4172-9079

Alasdair J. Nisbet https://orcid.org/0000-0002-4965-5396

José de la Fuente https://orcid.org/0000-0001-7383-9649

Article

Publisher ID: vaccines-07-00121

DOI: 10.3390/vaccines7030121

PMC ID: 6789658

PubMed ID: 31546944

SO-VID: 54c0c1d6-e287-47b9-9edc-3b75be6dc8ca

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( http://creativecommons.org/licenses/by/4.0/).

Reduction in Oviposition of Poultry Red Mite ( Dermanyssus gallinae) in Hens Vaccinated with Recombinant Akirin

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Abstract

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Malaria vaccine development

Most cited references 29

Tick vaccines: current status and future directions.

The tick protective antigen, 4D8, is a conserved protein involved in modulation of tick blood ingestion and reproduction.

Vaccination with proteins involved in tick-pathogen interactions reduces vector infestations and pathogen infection.

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