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      Production and characterization of galactose oxidase produced by four isolates of Fusarium graminearum

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          Abstract

          A screening aimed to find new galactose oxidase producer isolates and to evaluate the production among Fusarium graminearum strains was conducted. Thirty-five isolates out of 39 analysed produced the enzyme at several levels. The data indicated a wide distribution of galactose oxidase within F. graminearum and also revealed new producer isolates. The enzyme produced by different isolates showed similar thermal activity and stability and were active on same substrates. However, the optimum pH ranged from 7.0 to 7.5. Thus, all evaluated isolates were suitable for the production of galactose oxidase.

          Translated abstract

          Uma análise visando encontrar novos isolados produtores de galactose oxidase (GO) e avaliar a distribuição da produção entre cepas de Fusarium graminearum foi conduzida. Trinta e cinco isolados de 39 testados produziram a enzima em diferentes níveis. Os dados indicaram uma ampla distribuição da produção da galactose oxidase por F. graminearum e revelaram novos isolados produtores. A enzima produzida por diferentes isolados apresentou atividade e estabilidade térmicas similares e foi ativa sobre os mesmos substratos. No entanto, o pH ótimo variou de 7,0 a 7,5. Assim, todos os isolados são adequados para a produção de galactose oxidase.

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          Most cited references62

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          Free radical catalysis by galactose oxidase.

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            The genus Fusarium

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              The D-galactose oxidase of Polyporus circinatus.

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                Author and article information

                Journal
                babt
                Brazilian Archives of Biology and Technology
                Braz. arch. biol. technol.
                Instituto de Tecnologia do Paraná - Tecpar (Curitiba, PR, Brazil )
                1516-8913
                1678-4324
                July 2006
                : 49
                : 4
                : 557-564
                Affiliations
                [02] Maringá PR orgnameUniversidade Estadual de Maringá orgdiv1Departamento de Agronomia Brasil
                [01] orgnameUniversidade Estadual de Maringá orgdiv1Departamento de Bioquímica
                Article
                S1516-89132006000500004 S1516-8913(06)04900404
                10.1590/S1516-89132006000500004
                54b919dc-3c4d-4763-a7e9-60a1f920f21b

                This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

                History
                : 10 December 2004
                : 19 July 2005
                : 27 March 2006
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 36, Pages: 8
                Product

                SciELO Brazil

                Self URI: Full text available only in PDF format (EN)
                Categories
                Agriculture, Agribusiness and Biotechnology

                Galactose oxidase,Enzyme,Fusarium graminearum
                Galactose oxidase, Enzyme, Fusarium graminearum

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