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      Tomato chocolàte virus: a new plant virus infecting tomato and a proposed member of the genus Torradovirus

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          Abstract

          A new virus was isolated from a tomato plant from Guatemala showing necrotic spots on the bases of the leaves and chocolate-brown patches on the fruits. Structural and molecular analysis showed the virus to be clearly related to but distinct from the recently described Tomato torrado virus (ToTV) and Tomato marchitez virus (ToMarV), both members of the genus Torradovirus. The name tomato chocolàte virus is proposed for this new torradovirus.

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          TreeView: an application to display phylogenetic trees on personal computers.

          R D Page (1996)
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            Viral cysteine proteases are homologous to the trypsin-like family of serine proteases: structural and functional implications.

            Proteases that are encoded by animal picornaviruses and plant como- and potyviruses form a related group of cysteine-active-center enzymes that are essential for virus maturation. We show that these proteins are homologous to the family of trypsin-like serine proteases. In our model, the active-site nucleophile of the trypsin catalytic triad, Ser-195, is changed to a Cys residue in these viral proteases. The other two residues of the triad, His-57 and Asp-102, are otherwise absolutely conserved in all the viral protease sequences. Secondary structure analysis of aligned sequences suggests the location of the component strands of the twin beta-barrel trypsin fold in the viral proteases. Unexpectedly, the 2a and 3c subclasses of viral cysteine proteases are, respectively, homologous to the small and large structural subclasses of trypsin-like serine proteases. This classification allows the molecular mapping of residues from viral sequences onto related tertiary structures; we precisely identify amino acids that are strong determinants of specificity for both small and large viral cysteine proteases.
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              Secoviridae: a proposed family of plant viruses within the order Picornavirales that combines the families Sequiviridae and Comoviridae, the unassigned genera Cheravirus and Sadwavirus, and the proposed genus Torradovirus.

              The order Picornavirales includes several plant viruses that are currently classified into the families Comoviridae (genera Comovirus, Fabavirus and Nepovirus) and Sequiviridae (genera Sequivirus and Waikavirus) and into the unassigned genera Cheravirus and Sadwavirus. These viruses share properties in common with other picornavirales (particle structure, positive-strand RNA genome with a polyprotein expression strategy, a common replication block including type III helicase, a 3C-like cysteine proteinase and type I RNA-dependent RNA polymerase). However, they also share unique properties that distinguish them from other picornavirales. They infect plants and use specialized proteins or protein domains to move through their host. In phylogenetic analysis based on their replication proteins, these viruses form a separate distinct lineage within the picornavirales branch. To recognize these common properties at the taxonomic level, we propose to create a new family termed "Secoviridae" to include the genera Comovirus, Fabavirus, Nepovirus, Cheravirus, Sadwavirus, Sequivirus and Waikavirus. Two newly discovered plant viruses share common properties with members of the proposed family Secoviridae but have distinct specific genomic organizations. In phylogenetic reconstructions, they form a separate sub-branch within the Secoviridae lineage. We propose to create a new genus termed Torradovirus (type species, Tomato torrado virus) and to assign this genus to the proposed family Secoviridae.
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                Author and article information

                Contributors
                martin.verbeek@wur.nl
                Journal
                Arch Virol
                Archives of Virology
                Springer Vienna (Vienna )
                0304-8608
                1432-8798
                13 March 2010
                13 March 2010
                May 2010
                : 155
                : 5
                : 751-755
                Affiliations
                [1 ]Plant Research International, Part of Wageningen UR (University and Research Centre), Wageningen, The Netherlands
                [2 ]De Ruiter Seeds, Bergschenhoek, The Netherlands
                [3 ]Nickerson-Zwaan, Made, The Netherlands
                Article
                640
                10.1007/s00705-010-0640-1
                2861173
                20229114
                54248317-283c-4afb-bc51-216760eb1d28
                © The Author(s) 2010
                History
                : 7 January 2010
                : 19 February 2010
                Categories
                Brief Report
                Custom metadata
                © Springer-Verlag 2010

                Microbiology & Virology
                Microbiology & Virology

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