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      Nuevos registros de la ardilla de Peters Sciurus oculatus (Peters 1863) para Michoacán y primera genotipificación molecular de la especie Translated title: New records of Peters squirrel Sciurus oculatus (Peters 1863) for Michoacán and first molecular genotyping of the species

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          Abstract

          Capturamos tres ejemplares de la ardilla de Peters (Sciurus oculatus), especie listada en protección especial y endémica del centro de México. Las capturas ocurrieron en fragmentos de bosque de encino del municipio de Huandacareo, Michoacán. Con el registro de esta población se confirma la presencia actual de la especie en el estado, ya que su último registro es de 1986 de un ejemplar colectado en Contepec. La localidad de Huandacareo extiende la distribución conocida de la especie hacia el oeste en 48 km aproximadamente con respecto a su registro más reciente. Al revisar los pocos registros de la especie para el estado, se observó que el registro más occidental de la especie, el de Ziracuaretiro (cerca de Uruapan), no se incluyó en la delimitación de su distribución, por lo que el mapa de distribución conocida debe actualizarse incluyendo todas las localidades, lo que modificaría de forma importante su distribución. Revisando la base de datos de GeneBank, notamos la inexistencia de secuencias de ADN mitocondrial de S. oculatus. En este estudio presentamos las dos primeras secuencias del gen de citocromo b (cytb) provenientes de dos individuos de S. oculatus. Las secuencias fueron comparadas en análisis filogenético con ocho especies del género Sciurus disponibles en GeneBank. En el análisis de las secuencias y árbol de UPGMA que incluyó especies de Norteamérica y de Sudamerica, S. oculatus se agrupó con S. aestuans y S. stramineus de Sudamérica, siendo S. oculatus la especie basal. Es importante que en el futuro se investigue con mayor detalle la relación de la especie en el género Sciurus. Desde la perspectiva de conservación debe evaluarse su distribución actual en el país. Actualmente se desconoce cuántas poblaciones existen en áreas protegidas de México. La población registrada en Huandacareo parece estar aislada y su hábitat rodeado de agricultura.

          Translated abstract

          We captured three individuals of Peter's squirrel (Sciurus oculatus), species listed under special protection and endemic for central Mexico. The captures occurred on oak forest fragments from Huandacareo municipality, Michoacán. With the record of this population, we confirm the species actual presence for the state, because the last record corresponded to a specimen collected in Contepec in 1986. This locality extends the species known distribution to the west approximately in 48 km from its last known record. When we reviewed the species historical records for the state, we notice that the western-most record of the species, a record from Ziracuaretiro (near to Uruapan), was not included to outline the species global distribution, therefore we consider that the present distributional map should be updated including all the localities, which will modify its distribution considerably. After surveying the GeneBank international database, we noticed that mitocondrial DNA sequences from S. oculatus were inexistent. In this work we presented the first two cytochrome b gene (cytb) sequences from two S. oculatus individuals. The sequences were compared with eight species of the Sciurus genus available in GeneBank. In the sequences analysis and UPGMA tree, which included Sciurus species from North America and South America, S. oculatus was clustered with S. aestuans and S. stramineus from South America, resulting S. oculatus the basal species. From the conservation perspective, the species actual presencein the historical localities should be verified. No protected areas in the country are known to include Sciurus oculatus populations, and the Huandacareo recorded population seems to be relatively isolated, and its habitat surrounded by agriculture.

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          Most cited references23

          • Record: found
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          DNA sequencing with chain-terminating inhibitors

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            BioEdit a user-friendly biological sequences alignment editor and analysis program for Windows 9598NT

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              • Abstract: found
              • Article: not found

              Evolution of the cytochrome b gene of mammals.

              With the polymerase chain reaction (PCR) and versatile primers that amplify the whole cytochrome b gene (approximately 1140 bp), we obtained 17 complete gene sequences representing three orders of hoofed mammals (ungulates) and dolphins (cetaceans). The fossil record of some ungulate lineages allowed estimation of the evolutionary rates for various components of the cytochrome b DNA and amino acid sequences. The relative rates of substitution at first, second, and third positions within codons are in the ratio 10 to 1 to at least 33. For deep divergences (greater than 5 million years) it appears that both replacements and silent transversions in this mitochondrial gene can be used for phylogenetic inference. Phylogenetic findings include the association of (1) cetaceans, artiodactyls, and perissodactyls to the exclusion of elephants and humans, (2) pronghorn and fallow deer to the exclusion of bovids (i.e., cow, sheep, and goat), (3) sheep and goat to the exclusion of other pecorans (i.e., cow, giraffe, deer, and pronghorn), and (4) advanced ruminants to the exclusion of the chevrotain and other artiodactyls. Comparisons of these cytochrome b sequences support current structure-function models for this membrane-spanning protein. That part of the outer surface which includes the Qo redox center is more constrained than the remainder of the molecule, namely, the transmembrane segments and the surface that protrudes into the mitochondrial matrix. Many of the amino acid replacements within the transmembrane segments are exchanges between hydrophobic residues (especially leucine, isoleucine, and valine). Replacement changes at first and second positions of codons approximate a negative binomial distribution, similar to other protein-coding sequences. At four-fold degenerate positions of codons, the nucleotide substitutions approximate a Poisson distribution, implying that the underlying mutational spectrum is random with respect to position.
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                Author and article information

                Journal
                azm
                Acta zoológica mexicana
                Acta Zool. Mex
                Instituto de Ecología A.C. (Xalapa, Veracruz, Mexico )
                0065-1737
                2448-8445
                August 2013
                : 29
                : 2
                : 304-316
                Affiliations
                [03] D. F. orgnameUniversidad Nacional Autónoma de México orgdiv1Facultad de Ciencias orgdiv2Museo de Zoología Alfonso L. Herrera México
                [02] Morelia Michoacán orgnameUniversidad Michoacana de San Nicolás de Hidalgo orgdiv1Facultad de Medicina Veterinaria y Zootecnia orgdiv2Centro Multidisciplinario de Estudios en Biotecnología México
                [01] Morelia Michoacán orgnameUniversidad Michoacana de San Nicolás de Hidalgo orgdiv1Facultad de Biología orgdiv2Laboratorio de Ecología de Vertebrados Terrestres Prioritarios México tiberio@ 123456umich.mx
                Article
                S0065-17372013000200004 S0065-1737(13)02900200004
                5124028c-120c-44a4-b1eb-7a8cba4e2ffa

                This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

                History
                : 23 April 2013
                : 08 March 2012
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 23, Pages: 13
                Product

                SciELO Mexico

                Categories
                Artículos originales

                Sciuridae,distribución,endémica Mexicana,citocromo b,distribution,Mexican endemic,cytochrome b

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