Lee A. Armstrong:
ORCID: https://orcid.org/0000-0001-7036-625X
Role: ConceptualizationRole: InvestigationRole: Writing – review & editing
Sven M. Lange:
ORCID: https://orcid.org/0000-0002-6011-8647
Role: ConceptualizationRole: InvestigationRole: MethodologyRole: Writing – review
& editing
Virginia Dee Cesare:
ORCID: https://orcid.org/0000-0002-2640-6389
Role: Formal analysisRole: InvestigationRole: Writing – review & editing
Stephen P. Matthews: Role: ConceptualizationRole: Formal analysisRole: Investigation
Raja Sekhar Nirujogi: Role: Formal analysisRole: InvestigationRole: Writing – review & editing
Isobel Cole: Role: Investigation
Anthony Hope: Role: Formal analysis
Fraser Cunningham: Role: Formal analysisRole: Writing – review & editing
Rachel Toth: Role: InvestigationRole: Resources
Rukmini Mukherjee: Role: Investigation
Denisa Bojkova: Role: Investigation
Franz Gruber: Role: Resources
David Gray:
ORCID: https://orcid.org/0000-0001-9512-3828
Role: Funding acquisitionRole: SupervisionRole: Writing – review & editing
Paul G. Wyatt: Role: Writing – review & editing
Jindrich Cinatl: Role: Supervision
Ivan Dikic: Role: Funding acquisitionRole: SupervisionRole: Writing – review & editing
Paul Davies: Role: Project administrationRole: Writing – review & editing
Yogesh Kulathu:
ORCID: https://orcid.org/0000-0002-3274-1642
Role: ConceptualizationRole: Formal analysisRole: Funding acquisitionRole: SupervisionRole:
Writing – original draftRole: Writing – review & editing
A Ganesan: Role: Editor
Journal ID (nlm-ta): PLoS One
Journal ID (iso-abbrev): PLoS One
Journal ID (publisher-id): plos
Title:
PLoS ONE
Publisher:
Public Library of Science
(San Francisco, CA USA
)
ISSN
(Electronic):
1932-6203
Publication date
(Electronic):
16
July
2021
Publication date Collection: 2021
Publication date PMC-release: 16
July
2021
Volume: 16
Issue: 7
Electronic Location Identifier: e0253364
Affiliations
[1
]
MRC Protein Phosphorylation and Ubiquitylation Unit, Sir James Black Centre, School
of Life Sciences, University of Dundee, Dundee, Scotland, United Kingdom
[2
]
Drug Discovery Unit, School of Life Sciences, University of Dundee, Dundee, Scotland,
United Kingdom
[3
]
MRC Reagents and Services, University of Dundee, Dundee, Scotland, United Kingdom
[4
]
Institute of Biochemistry II, Goethe University Frankfurt Medical Faculty, University
Hospital, Frankfurt am Main, Germany
[5
]
Buchmann Institute for Molecular Life Sciences, Goethe University Frankfurt, Frankfurt
am Main, Germany
[6
]
Institute of Medical Virology, University Hospital Frankfurt, Frankfurt am Main, Germany
[7
]
National Phenotypic Screening Centre, University of Dundee, Dundee, Scotland, United
Kingdom
[8
]
Max Planck Institute of Biophysics, Frankfurt am Main, Germany
University of East Anglia, UNITED KINGDOM
Author notes
Competing Interests: The authors have declared that no competing interests exist.
Author information
Article
Publisher ID:
PONE-D-21-09090
DOI: 10.1371/journal.pone.0253364
PMC ID: 8284666
PubMed ID: 34270554
SO-VID: 4fccf2d1-a9e7-4f2d-9d34-de099afbb363
Copyright © © 2021 Armstrong et al
License:
This is an open access article distributed under the terms of the
Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided
the original author and source are credited.
Funded by:
funder-id http://dx.doi.org/10.13039/100010663, h2020 european research council;
Award ID: 677623
Award Recipient
:
Stephen P. Matthews
Funded by:
funder-id http://dx.doi.org/10.13039/100010663, h2020 european research council;
Award ID: 677623
Award Recipient
:
ORCID: https://orcid.org/0000-0002-3274-1642
Yogesh Kulathu
Funded by:
medical research council
Award ID: MC_UU_00018/3
Award Recipient
:
ORCID: https://orcid.org/0000-0001-7036-625X
Lee A. Armstrong
Funded by:
medical research council
Award ID: MC_UU_00018/3
Award Recipient
:
ORCID: https://orcid.org/0000-0002-6011-8647
Sven M. Lange
Funded by:
medical research council
Award ID: MC_UU_00018/3
Award Recipient
:
ORCID: https://orcid.org/0000-0002-2640-6389
Virginia Dee Cesare
Funded by:
medical research council
Award ID: MC_UU_00018/3
Award Recipient
:
Stephen P. Matthews
Funded by:
medical research council
Award ID: MC_UU_00018/3
Award Recipient
:
Raja Sekhar Nirujogi
Funded by:
medical research council
Award ID: MC_UU_00018/3
Award Recipient
:
Isobel Cole
Funded by:
medical research council
Award ID: MC_UU_00018/3
Award Recipient
:
Rachel Toth
Funded by:
medical research council
Award ID: MC_UU_00018/3
Award Recipient
:
Paul Davies
Funded by:
medical research council
Award ID: MC_UU_00018/3
Award Recipient
:
ORCID: https://orcid.org/0000-0002-3274-1642
Yogesh Kulathu
Funded by:
funder-id http://dx.doi.org/10.13039/501100000723, tenovus;
Award Recipient
:
ORCID: https://orcid.org/0000-0001-7036-625X
Lee A. Armstrong
Funded by:
funder-id http://dx.doi.org/10.13039/501100000723, tenovus;
Award Recipient
:
ORCID: https://orcid.org/0000-0002-6011-8647
Sven M. Lange
Funded by:
funder-id http://dx.doi.org/10.13039/501100000723, tenovus;
Award Recipient
:
Stephen P. Matthews
Funded by:
funder-id http://dx.doi.org/10.13039/501100000723, tenovus;
Award Recipient
:
ORCID: https://orcid.org/0000-0002-3274-1642
Yogesh Kulathu
Funded by:
medical research council
Award ID: MC_PC_18044
Award Recipient
:
Anthony Hope
Funded by:
medical research council
Award ID: MC_PC_18044
Award Recipient
:
Fraser Cunningham
Funded by:
medical research council
Award ID: MC_PC_18044
Award Recipient
:
ORCID: https://orcid.org/0000-0001-9512-3828
David Gray
Funded by:
medical research council
Award ID: MC_PC_18044
Award Recipient
:
Paul G. Wyatt
Funded by:
funder-id http://dx.doi.org/10.13039/501100001255, lister institute of preventive medicine;
Award Recipient
:
ORCID: https://orcid.org/0000-0002-3274-1642
Yogesh Kulathu
Funded by:
funder-id http://dx.doi.org/10.13039/100004410, european molecular biology organization;
Award ID: EMBO Young Investigator Programme
Award Recipient
:
ORCID: https://orcid.org/0000-0002-3274-1642
Yogesh Kulathu
Funded by:
funder-id http://dx.doi.org/10.13039/100010663, h2020 european research council;
Award ID: 742720
Award Recipient
:
Rukmini Mukherjee
Funded by:
funder-id http://dx.doi.org/10.13039/100010663, h2020 european research council;
Award ID: 742720
Award Recipient
:
Ivan Dikic
UKRI | Medical Research Council (MRC):Lee A Armstrong,Sven M Lange,Virginia de Cesare,Stephen
P Matthews,Raja Sekar Nirujogi,Isobel Cole,Rachel Toth,Paul Davies,Yogesh Kulathu
MC_UU_00018/3; EC | H2020 | H2020 Priority Excellent Science | H2020 European Research
Council (ERC):Lee A Armstrong,Sven M Lange,Stephen P Matthews,Yogesh Kulathu 677623;
Tenovus:Lee A Armstrong,Sven M Lange,Stephen P Matthews,Yogesh Kulathu; UKRI | MRC
| Medical Research Foundation:Anthony Hope,Fraser Cunningham,David Gray,Paul G. Wyatt
MC_PC_18044; Lister Institute of Preventive Medicine:Yogesh Kulathu; EC | H2020 |
H2020 Priority Excellent Science | H2020 European Research Council (ERC):Rukmini Mukherjee,Ivan
Dikic 742720.
Subject:
Research Article
Subject:
Biology and Life Sciences
Subject:
Biochemistry
Subject:
Enzymology
Subject:
Enzymes
Subject:
Proteases
Subject:
Biology and Life Sciences
Subject:
Biochemistry
Subject:
Proteins
Subject:
Enzymes
Subject:
Proteases
Subject:
Biology and life sciences
Subject:
Organisms
Subject:
Viruses
Subject:
RNA viruses
Subject:
Coronaviruses
Subject:
SARS coronavirus
Subject:
SARS CoV 2
Subject:
Biology and life sciences
Subject:
Microbiology
Subject:
Medical microbiology
Subject:
Microbial pathogens
Subject:
Viral pathogens
Subject:
Coronaviruses
Subject:
SARS coronavirus
Subject:
SARS CoV 2
Subject:
Medicine and health sciences
Subject:
Pathology and laboratory medicine
Subject:
Pathogens
Subject:
Microbial pathogens
Subject:
Viral pathogens
Subject:
Coronaviruses
Subject:
SARS coronavirus
Subject:
SARS CoV 2
Subject:
Biology and life sciences
Subject:
Organisms
Subject:
Viruses
Subject:
Viral pathogens
Subject:
Coronaviruses
Subject:
SARS coronavirus
Subject:
SARS CoV 2
Subject:
Biology and Life Sciences
Subject:
Organisms
Subject:
Eukaryota
Subject:
Fungi
Subject:
Yeast
Subject:
Biology and Life Sciences
Subject:
Biochemistry
Subject:
Peptides
Subject:
Polypeptides
Subject:
Physical Sciences
Subject:
Chemistry
Subject:
Analytical Chemistry
Subject:
Mass Spectrometry
Subject:
Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry
Subject:
Research and Analysis Methods
Subject:
Spectrum Analysis Techniques
Subject:
Mass Spectrometry
Subject:
Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry
Subject:
Biology and Life Sciences
Subject:
Molecular Biology
Subject:
Molecular Biology Techniques
Subject:
Cloning
Subject:
Research and Analysis Methods
Subject:
Molecular Biology Techniques
Subject:
Cloning
Subject:
Biology and Life Sciences
Subject:
Microbiology
Subject:
Virology
Subject:
Viral Replication
Subject:
Research and Analysis Methods
Subject:
Electrophoretic Techniques
Subject:
Gel Electrophoresis
Subject:
Electrophoretic Staining
Subject:
Silver Staining
Subject:
Research and Analysis Methods
Subject:
Specimen Preparation and Treatment
Subject:
Staining
Subject:
Electrophoretic Staining
Subject:
Silver Staining