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      miR-27b-3p suppresses cell proliferation, migration and invasion by targeting LIMK1 in colorectal cancer

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          Abstract

          Accumulating evidence has indicated that microRNAs (miRNAs) can act as candidate tumor suppressors. However, the mechanism of miR-27b-3p in colorectal cancer (CRC) is still unknown. In this study, the relative expression levels of miR-27b-3p and LIMK1 were detected by quantitative real-time PCR (qRT-PCR) in CRC and adjacent normal tissues as well as in CRC cell lines. The proliferation, cell cycle and migratory and invasive abilities of SW620 cells were analyzed by CCK-8, flow cytometry, wound healing assays, and Transwell assays, respectively. The regulatory relationships between LIMK1 and miR-27b-3p were detected by qRT-PCR, luciferase reporter gene and Western blot assays. The results showed that the expression levels of miR-27b-3p were downregulated in CRC samples compared with those in adjacent nontumor tissues. Moreover, we found that miR-27b-3p suppressed CRC cell proliferation, cycle and migration and invasion. Additionally, there was an inverse correlation between LIMK1 expression and miR-27b-3p expression in CRC tissues. LIMK1 was identified as a direct target of miR-27b-3p. Our findings indicated that miR-27b-3p inhibited CRC cell proliferation, migration and invasion by targeting LIMK1.

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          Author and article information

          Journal
          Int J Clin Exp Pathol
          Int J Clin Exp Pathol
          ijcep
          International Journal of Clinical and Experimental Pathology
          e-Century Publishing Corporation
          1936-2625
          2017
          01 September 2017
          : 10
          : 9
          : 9251-9261
          Affiliations
          [1 ] Department of General Surgery, Tongde Hospital of Zhejiang Province Hangzhou, China
          [2 ] Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Military Sciences Beijing, China
          Author notes
          Address correspondence to: Ye Jin, Department of General Surgery, Tongde Hospital of Zhejiang Province, 234 Gucui Road, Xihu District, Hangzhou 310012, China. Tel: +86-571-89972361; Fax: 86-571-89972361; E-mail: yejin_tjmu@ 123456tom.com
          Article
          PMC6966001 PMC6966001 6966001
          6966001
          31966797
          4eab0f67-66d8-4cac-ba29-046499e30aa7
          IJCEP Copyright © 2017
          History
          : 24 May 2017
          : 20 July 2017
          Categories
          Original Article

          proliferation,invasion,migration,colorectal cancer,LIMK1,miR-27b-3p

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