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      Development and Validation of HPLC Method for Quantification of Plumbagin in Plumbago Zeylanica L. Roots

      Author(s): 1 , 1
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      Journal: Drug Research

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          Abstract

          Background An RP-HPLC (Reverse Phase-High-performance liquid chromatography) method for the quantitative estimation and validation of the plumbagin in the methanolic fraction of Plumbago zeylanica L. was developed.

          Method For achieving good separation, the RP-HPLC method was carried out with reverse phase C18 column, using methanol and water as mobile phase in the ratio of 65:35 (v/v), at the flow rate of 1 mL/min. The detection wavelength was set at 265 nm.

          Results The retention time of plumbagin was found at 7.5±0.2 min. The coefficient of determination of plumbagin was found to be (r2) 0.9985 and equation Y=23148x+4327. The LOD and LOQ were found to be 34.06 and 113 ng/mL, respectively.

          Conclusion The developed method was accurate, specific, precise, and reproducible. This RP-HPLC may be useful for quantitative estimation of the chemical constituents present in the plant extract as well as the quality assessment of the herbal product.

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          RP-HPLC analysis of methanol extract of Viscum articulatum

          Mrinmoy Nag, Amit Kar, Joydeb Chanda … (2019)
          Background Viscum articulatum Burm. (Family: Loranthaceae) is commonly known as mistletoe. In ayurveda, the plant parts are used in “Kapha”, “Vata”, diseases of the blood, ulcer, and epilepsy. The plant parts are also used in urinary tract infection and wound infection. The plant contains five triterpenoids such as α-amyrin, lupeol, betulin, betulinic acid and oleanolic acid, exhibiting several pharmacological activities including antimicrobial, anti-HIV, antitumor, antiviral activity. Objective To ensure the content of uniformity of oleanolic acid, a RP-HPLC method has been developed for estimation of oleanolic acid in V. articulatum aerial part. Material and methods The RP-HPLC method was carried out in reverse phase C18 column, using methanol and water as mobile phase in the ratio of 95:5 (v/v), at the flow rate of 1 mL/min. The pH of aqueous phase was adjusted 3.2 with 1% (v/v) glacial acetic acid. The λ max was set at 210 nm. Results The retention time of oleanolic acid was found at 21.5 ± 0.05 min. The linearity of the response was found to be 10–800 μg/mL. The coefficient of determinants of oleanolic acid was found to be (r2) 0.995 and equation Y = 19462X + 16,172. The LOD and LOQ were found to be for oleanolic acid (1.96% w/w) 0.197 ± 0.63 and 0.623 ± 0.87 μg/mL, respectively. The developed method was accurate, specific, precise and reproducible. Conclusion This RP-HPLC may be useful for quantitative estimation of the chemical constituents present in the plant extract as well as the quality assessment of the herbal product.
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            Phytochemical evaluation of roots of Plumbago zeylanica L. and assessment of its potential as a nephroprotective agent

            R Rajakrishnan, R Lekshmi, P.B. Benil … (2017)
            Search for medicinal plants to treat kidney disorders is an important topic on phytotherapeutical research. Plumbago zeylanica L. is an important medicinal plant with hepatoprotective, anti-inflammatory, anti-diabetic, anti-cancer and anti-hyperlipidemic activities. In the present study, the protective effect of hydroalcoholic extract of P. zeylanica (HAPZ) in cisplatin induced nephrotoxicity was analyzed in Swiss albino mice. Treatment with higher dose (400 mg/kg) of HAPZ significantly reversed the adverse effect of cisplatin on kidney weight, serum urea and creatinine, indicating their renoprotective effect. The antioxidant effect of the drug is evident from its significant effect on Catalase, Glutathione peroxidase and lipid peroxidation activities.
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              High-Performance Liquid Chromatography (HPLC) as a Tool for Standardization of Complex Herbal Drugs.

              Raghavan Govindarajan, Vyas Tejas, Palpu Pushpangadan (2019)
              Background: Herbal formulations have reached tremendous acceptability as therapeutic agents for several diseases mainly due to the indiscriminate use of modern medicine such as antibiotics, steroids and other synthetic drugs. The increasing popularity in plant-based drugs is leading to a fast growing market for plant-based drugs pharmaceuticals, nutraceuticals, functional foods, and even cosmeceuticals. Objective: The development of authentic analytical methods for complex herbal drugs, especially poly herbal formulations which can reliably profile the phytochemical composition, including quantitative analyses of marker/bioactive compounds and other major constituents, is a major challenge to scientists. Standardization is an important step for the establishment of a consistent biological activity, a consistent chemical profile, or simply a quality assurance program for production and manufacturing of herbal drugs. Methods/Results: HPLC as a tool has been widely used in the standardization of complex herbal drugs because of its ability to estimate the presence of active (chemical or biological) markers both qualitatively and quantitatively. Conclusions: An overview of various HPLC techniques that can be used for standardization of herbal drugs has been presented.
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                Author and article information

                Journal
                Title: Drug Research
                Abbreviated Title: Drug Res (Stuttg)
                ISSN (Print): 2194-9379
                ISSN (Electronic): 2194-9387
                Publication date Created: April 03 2023
                Publication date Created: April 2023
                Publication date (Electronic): February 23 2023
                Publication date (Print): April 2023
                Volume: 73
                Issue: 04
                Pages: 238-242
                Affiliations
                [1 ]Faculty of Pharmacy, Integral University, Lucknow, India
                Article
                DOI: 10.1055/a-2019-4985
                SO-VID: 4e2699da-a938-4eb5-9833-409f7cec5b47
                Copyright © © 2023
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