Altered microbial diversity and composition of multiple mucosal organs in cervical cancer patients

Abstract

Objectives

The aim of this study was to characterize the microbiome of multiple mucosal organs in cervical cancer (CC) patients.

Methods

We collected oral, gut, urinary tract, and vaginal samples from enrolled study participants, as well as tumor tissue from CC patients. The microbiota of different mucosal organs was identified by 16S rDNA sequencing and correlated with clinical-pathological characteristics of cervical cancer cases.

Results

Compared with controls, CC patients had reduced α-diversity of oral and gut microbiota ( p _{Oral_Sob} < 0.001, p _{Oral_Shannon} = 0.049, p _{Oral_Simpson} = 0.013 p _{Fecal_Sob} = 0.030), although there was an opposite trend in the vaginal microbiota ( p _{Vaginal_Pielou} = 0.028, p _{Vaginal_Simpson} = 0.006). There were also significant differences in the β-diversity of the microbiota at each site between cases and controls ( p _Oral = 0.002, p _Fecal = 0.037, p _Urine = 0.001, p _Vaginal = 0.001). The uniformity of urine microbiota was lower in patients with cervical squamous cell carcinoma ( p _Urine = 0.036) and lymph node metastasis ( p _{Urine_Sob} = 0.027, p _{Urine_Pielou} = 0.028, p _{Urine_Simpson} = 0.021, p _{Urine_Shannon} = 0.047). The composition of bacteria in urine also varied among patients with different ages ( p = 0.002), tumor stages ( p = 0.001) and lymph node metastasis ( p = 0.002). In CC cases, Pseudomonas were significantly enriched in the oral, gut, and urinary tract samples. In addition, Gardnerella, Anaerococcus, and Prevotella were biomarkers of urinary tract microbiota; Abiotrophia and Lautropia were obviously enriched in the oral microbiota. The microbiota of tumor tissue correlated with other mucosal organs (except the gut), with a shift in the microflora between mucosal organs and tumors.

Conclusions

Our study not only revealed differences in the composition and diversity of the vaginal and gut microflora between CC cases and controls, but also showed dysbiosis of the oral cavity and urethra in cervical cancer cases.

Supplementary Information

The online version contains supplementary material available at 10.1186/s12885-024-12915-1.