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      Use of dermal-fat grafts in the post-oncological reconstructive surgery of atrophies in the zygomatic region: clinical evaluations in the patients undergone to previous radiation therapy

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          Abstract

          Introduction

          Grafting of autologous adipose tissue can be recommended in some cases of facial plastic surgery. Rhabdomyosarcoma is a type of cancer that can also affect the orbit. Enucleation of the eye can cause atrophy of the corresponding hemiface and decreased orbital growth.

          Case report

          We report a case of a female patient with a medical history of surgical enucleation of the right eyeball, who had received rhabdomyosarcoma radiation therapy in her youth. The patient presented with a depression in the right zygomatic region. We took a dermal-fat flap from the abdominal region, which had been previously treated.

          Results

          The surgical outcome, 48 hours, and much clearly 31 days after the surgery, revealed that the right zygomatic region had returned to its proper anatomical shape, although there were still signs of postoperative edema.

          Discussion

          Very damaged tissues, like those exposed to radiation therapy, are generally not suitable for grafting of adipose tissue.

          Conclusions

          In the described case, we achieved a technically and aesthetically satisfying result despite the patient's medical history involving several perplexities about the use of autologous dermal-fat tissues, because of prior radiation therapy exposure. The clinical case shows that even a region exposed to radiation therapy can be a valid receiving bed for dermal-fat grafting.

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          Most cited references21

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          Facial recontouring with lipostructure.

          Lipostructure is a natural, long-lasting method of filling and supporting the face using intricate layering of infiltrated autologous tissue. This method allows the tissues to be sculpted to enact three-dimensional augmentation of facial elements. Because the grafted fat becomes integrated into the host tissues, it is almost undetectable after transplantation, except by photography. To successfully use fatty tissue as such a graft, attention must be paid to the nature of fatty tissue; to the methods of harvesting, transfer, and placement; and to the preparation of the patient. Fatty tissue is a complex, delicate structure that is easily damaged by mechanical and chemical insults. Successful fat transplantation demands that every step be practiced with attention to this fragile nature of fatty tissue. Precision is an important consideration in the augmentation of millimeters of facial elements. The true volume of infiltration is difficult to judge if too much blood, lidocaine, or oil is present in the tissue being placed. Fat is living tissue that must be in close proximity to a nutritional and respiratory source to survive. Therefore, placement of small amounts of fatty tissue in multiple tunnels assumes the utmost importance in the quest for both survival of fatty tissue and an aesthetically appropriate correction. Successful, three-dimensional sculpting requires attention to patient preparation, meticulous planning, and fastidious photographic evaluation. The potential applications in aesthetic and reconstructive surgeries of this new tool are profound. Lipostructure represents an important advance in plastic surgery: a safe, long-lasting method of recontouring the face with autologous tissue.
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            Autologous human fat grafting: effect of harvesting and preparation techniques on adipocyte graft survival.

            Autogenous fat transfer with lipoinjection for soft-tissue augmentation is a commonly used technique without a universally accepted approach. The high percentage and variable amount of fat resorption reduce the clinical efficacy of this procedure and often result in the need for further grafting. The purposes of this study were to evaluate the effect of different harvesting and preparation techniques on human fat tissue viability and to determine fat tissue viability rates among the different fat preparations transplanted into a severe combined immune deficiency mouse model at 3 months. Using standard liposuction and syringe aspiration, fat was removed from patients (n = 3) undergoing elective body contouring. Tissue was prepared by six different combinations of centrifugation and/or washing the cells with lactated Ringer's solution or normal saline. Metabolic activities of fat cell viability were monitored to assess overall cell viability. To analyze viability over 3 months, freshly harvested tissue specimens (minimum n = 5) were prepared by a combination of various procedures (wash, centrifugation, and different solutions) and subsequently injected under the dorsal flank skin of severe combined immune deficiency mice in two experiments. Mice were monitored for 12 weeks and the fat xenografts were removed for mass and histological evaluations. Metabolic analyses showed improved cell viability in tissue specimens undergoing minimal manipulation. No significant differences in fat cell viability, as assessed by graft weight maintenance or histologic evaluations, were observed with regard to harvesting or preparation techniques. Improved viability of freshly harvested but untreated fat specimens may be expected as compared with grafts that have undergone additional manipulations. No unique combination of preparation or harvesting techniques appeared to be more advantageous on transplanted fat grafts at 3 months. This study also demonstrated a reliable animal model for future investigation into examining novel applications for augmenting fat graft survival.
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              Comparative study of survival of autologous adipose tissue taken and transplanted by different techniques.

              In recent years, adipocytes obtained by suction-assisted lipectomy have been used for implantation by injection methods. This study is designed to assess the appearance of suctioned and excised adipose tissue and its survival after being injected or implanted into different tissues (0.5 cc into the rectus muscle and 0.5 cc into the dorsal ear skin) of New Zealand White rabbits. The results showed that significant numbers of adipocytes were ruptured after suction procedures. The intact cells represented approximately 10 percent of the fat cell population. Fat cells in aspirated and excised samples remained intact and did not differ histologically. After being injected into tissue, adipocytes appeared to survive better for a short term in a more vascularized bed (rectus muscle) than in a low vascular area (ear dermis). Long-term studies at 6- to 9-month intervals revealed transplanted adipose tissue, taken by suction or excision, being replaced with fibrosis, although cystic spaces and only a small number of surviving adipocytes were still present. Insulin did not show any protective effects on survival of the adipocytes during their transplantation.
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                Author and article information

                Journal
                Head Face Med
                Head Face Med
                Head & Face Medicine
                BioMed Central
                1746-160X
                2012
                5 December 2012
                : 8
                : 33
                Affiliations
                [1 ]Department of Dental Sciences and Surgery, University of Bari, Bari, Italy
                [2 ]Department of Medical Biochemistry, Medical Biology and Physics, University of Bari, Bari, Italy
                [3 ]Department of “Head and Neck Diseases”, Hospital “Fatebenefratelli”, Rome, Italy
                [4 ]Department of Maxillofacial Surgery, Calabrodental, Crotone, Italy
                [5 ]School of Dentistry, University of Bari, Bari, Italy
                [6 ]Department of Surgical, Reconstructive and Diagnostic Sciences, University of Milano, Milan, Italy
                [7 ]Department of Stomatology and Maxillofacial Sciences, University of Rome "Sapienza", Rome, Italy
                [8 ]Department of Clinical Sciences and Translational Medicine, University of Rome "Tor Vergata", Rome, Italy
                [9 ]Tecnologica Research Institute, Regenerative Medicine Section, Street E. Fermi, Crotone, Italy
                Article
                1746-160X-8-33
                10.1186/1746-160X-8-33
                3527323
                23217096
                4c53f97e-5dcf-4a54-acc0-a9b30811e6ab
                Copyright ©2012 Inchingolo et al.; licensee BioMed Central Ltd.

                This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

                History
                : 22 October 2012
                : 27 November 2012
                Categories
                Short Report

                Orthopedics
                dermal-fat grafts,oncological surgery,plastic surgery
                Orthopedics
                dermal-fat grafts, oncological surgery, plastic surgery

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