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      • Record: found
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      Identification and functional analysis of cation-efflux transporter 1 from Brassica juncea L.

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          Abstract

          Background

          Brassica juncea behaves as a moderate-level accumulator of various heavy metal ions and is frequently used for remediation. To investigate the roles of metal ion transporters in B. juncea, a cation-efflux family gene, BjCET1, was cloned and functionally characterized.

          Results

          BjCET1 contains 382 amino acid residues, including a signature motif of the cation diffusion facilitator protein family, six classic trans-membrane-spanning structures and a cation-efflux domain. A phylogenetic analysis showed that BjCET1 has a high similarity level with metal tolerance proteins from other Brassica plants, indicating that this protein family is highly conserved in Brassica. BjCET1 expression significantly increased at very early stages during both cadmium and zinc treatments. Green fluorescence detection in transgenic tobacco leaves revealed that BjCET1 is a plasma membrane-localized protein. The heterologous expression of BjCET1 in a yeast mutant increased the heavy-metal tolerance and decreased the cadmium or zinc accumulations in yeast cells, suggesting that BjCET1 is a metal ion transporter. The constitutive expression of BjCET1 rescued the heavy-metal tolerance capability of transgenic tobacco plants.

          Conclusions

          The data suggest that BjCET1 is a membrane-localized efflux transporter that plays essential roles in heavy metal ion homeostasis and hyper-accumulation.

          Supplementary Information

          The online version contains supplementary material available at 10.1186/s12870-022-03569-x.

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          Most cited references40

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          Cellular mechanisms for heavy metal detoxification and tolerance

          J.L. Hall (2002)
          Article 0 comments Cited 363 times – based on 0 reviews     Review now
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            Cadmium stress in rice: toxic effects, tolerance mechanisms, and management: a critical review.

            Muhammad Rizwan, Shafaqat Ali, Muhammad Nawaz Adrees … (2016)
            Cadmium (Cd) is one of the main pollutants in paddy fields, and its accumulation in rice (Oryza sativa L.) and subsequent transfer to food chain is a global environmental issue. This paper reviews the toxic effects, tolerance mechanisms, and management of Cd in a rice paddy. Cadmium toxicity decreases seed germination, growth, mineral nutrients, photosynthesis, and grain yield. It also causes oxidative stress and genotoxicity in rice. Plant response to Cd toxicity varies with cultivars, growth condition, and duration of Cd exposure. Under Cd stress, stimulation of antioxidant defense system, osmoregulation, ion homeostasis, and over production of signaling molecules are important tolerance mechanisms in rice. Several strategies have been proposed for the management of Cd-contaminated paddy soils. One such approach is the exogenous application of hormones, osmolytes, and signaling molecules. Moreover, Cd uptake and toxicity in rice can be decreased by proper application of essential nutrients such as nitrogen, zinc, iron, and selenium in Cd-contaminated soils. In addition, several inorganic (liming and silicon) and organic (compost and biochar) amendments have been applied in the soils to reduce Cd stress in rice. Selection of low Cd-accumulating rice cultivars, crop rotation, water management, and exogenous application of microbes could be a reasonable approach to alleviate Cd toxicity in rice. To draw a sound conclusion, long-term field trials are still required, including risks and benefit analysis for various management strategies.
            Article 0 comments Cited 162 times – based on 0 reviews     Review now
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              The Arabidopsis metal tolerance protein AtMTP3 maintains metal homeostasis by mediating Zn exclusion from the shoot under Fe deficiency and Zn oversupply.

              Toralf Senger, StÉphanie Arrivault, Ute Krämer (2006)
              Zinc ions are required to maintain the biological activity of numerous proteins. However, when mislocalized or accumulated in excess, Zn(2+) ions are toxic because of adventitious binding to proteins and displacement of other metal ions, among them Fe(2+), from their binding sites. Heterologous expression of a previously uncharacterized Arabidopsis thaliana metal tolerance protein, MTP3, in the zrc1 cot1 mutant of budding yeast restores tolerance to, and cellular accumulation of, zinc and cobalt. An MTP3-GFP fusion protein localizes to the vacuolar membrane when expressed in Arabidopsis. Ectopic over-expression of MTP3 increases Zn accumulation in both roots and rosette leaves of A. thaliana, and enhances Zn tolerance. Exposure of wild-type plants to high but non-toxic concentrations of Zn or Co, or Fe deficiency, strongly induce MTP3 expression specifically in epidermal and cortex cells of the root hair zone. Silencing of MTP3 by RNA interference causes Zn hypersensitivity and enhances Zn accumulation in above-ground organs of soil-grown plants and of seedlings exposed to excess Zn or to Fe deficiency. Our data indicate that, in wild-type A. thaliana, the AtMTP3 protein contributes to basic cellular Zn tolerance and controls Zn partitioning, particularly under conditions of high rates of Zn influx into the root symplasm.
              Article 0 comments Cited 119 times – based on 0 reviews     Review now
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                Author and article information

                Contributors
                Lu Han: edith1223@126.com
                Xiaohua Wu: huanongying1985@163.com
                Xinyu Zhang: 1721610968@qq.com
                Kailin Hou: h804669836@163.com
                Hongshan Zhang: zhanghs3@stu.hznu.edu.cn
                Chenjia Shen: shencj@hznu.edu.cn
                Journal
                Journal ID (nlm-ta): BMC Plant Biol
                Journal ID (iso-abbrev): BMC Plant Biol
                Title: BMC Plant Biology
                Publisher: BioMed Central (London )
                ISSN (Electronic): 1471-2229
                Publication date (Electronic): 6 April 2022
                Publication date PMC-release: 6 April 2022
                Publication date Collection: 2022
                Volume: 22
                Electronic Location Identifier: 174
                Affiliations
                [1 ]GRID grid.410595.c, ISNI 0000 0001 2230 9154, College of Life and Environmental Sciences, , Hangzhou Normal University, ; Hangzhou, 310036 China
                [2 ]GRID grid.410595.c, ISNI 0000 0001 2230 9154, Zhejiang Key Laboratory of Organ Development and Regeneration, College of Life and Environmental Sciences, Hangzhou Normal University, ; Hangzhou, 310036 Zhejiang China
                [3 ]GRID grid.410595.c, ISNI 0000 0001 2230 9154, Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, ; Hangzhou, 310036 China
                Article
                Publisher ID: 3569
                DOI: 10.1186/s12870-022-03569-x
                PMC ID: 8985314
                PubMed ID: 34979920
                SO-VID: 4b53fe4b-113b-4465-93a1-152071aefaa2
                Copyright © © The Author(s) 2022
                License:

                Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

                History
                Date received : 26 October 2021
                Date accepted : 31 March 2022
                Categories
                Subject: Research
                Custom metadata
                issue-copyright-statement © The Author(s) 2022

                ScienceOpen disciplines: Plant science & Botany
                Keywords: brassica juncea,cation-efflux transporter,heavy metal ion,heavy metal tolerance,heterologous expression
                Data availability:
                ScienceOpen disciplines: Plant science & Botany
                Keywords: brassica juncea, cation-efflux transporter, heavy metal ion, heavy metal tolerance, heterologous expression

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