Impact of human adipose tissue-derived stem cells on dermatofibrosarcoma protuberans cells in an indirect co-culture: an in vitro study

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Abstract

Background

Autologous adipose tissue transfer may be performed for aesthetic needs following the resection of dermatofibrosarcoma protuberans (DFSP), the most common cutaneous soft tissue sarcoma, excluding Kaposi sarcoma. The regenerative effectiveness of cell-assisted lipotransfer is dependent on the presence of adipose tissue-derived stem cells (ADSCs). This is the first study to evaluate the potential oncological risks as ADSCs could unintentionally be sited within the proximity of the tumor microenvironment of DFSP cells.

Methods

Primary DFSP cells were indirectly co-cultured with ADSCs in a conditioned medium or in a Transwell system. The impact was analyzed by assessing proliferation, migration, invasion, angiogenesis, and tumor-associated genes and proteins. Results of these assays were compared between co-culture and mono-culture conditions.

Results

Our experimental results showed that ADSCs were able to promote proliferation, migration, invasion, and angiogenesis of DFSP cells; this was accompanied by a significant increase in the expression levels of beta-type platelet-derived growth factor receptor, collagen type I alpha 1 chain, vascular endothelial growth factor, hepatocyte growth factor, and basic fibroblast growth factor.

Conclusions

The current report clearly demonstrates that ADSCs can enhance different malignant properties of DFSP cells in vitro, which should not be neglected when considering the clinical use of human ADSCs and its related derivatives in skin regenerative therapies.

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Most cited references 72

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Author and article information

Contributors

Fei Liu: Plasticliu@hotmail.com

Di Sun: sundi1980hn@sina.com

Journal

Journal ID (nlm-ta): Stem Cell Res Ther

Journal ID (iso-abbrev): Stem Cell Res Ther

Title: Stem Cell Research & Therapy

Publisher: BioMed Central (London )

ISSN (Electronic): 1757-6512

Publication date (Electronic): 6 August 2021

Publication date PMC-release: 6 August 2021

Publication date Collection: 2021

Volume: 12

Electronic Location Identifier: 440

Affiliations

[1 ]GRID grid.16821.3c, ISNI 0000 0004 0368 8293, Department of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, , Shanghai Jiao Tong University School of Medicine, ; Shanghai, 200011 China

[2 ]GRID grid.16821.3c, ISNI 0000 0004 0368 8293, Shanghai Key Lab of Tissue Engineering, Shanghai Ninth People’s Hospital, , Shanghai Jiao Tong University School of Medicine, ; Shanghai, 200011 China

[3 ]GRID grid.16821.3c, ISNI 0000 0004 0368 8293, Department of Oral and Cranio-maxillofacial Surgery, Shanghai Ninth People’s Hospital, College of Stomatology, , Shanghai Jiaotong University School of Medicine, ; Shanghai, 200011 China

Article

Publisher ID: 2512

DOI: 10.1186/s13287-021-02512-5

PMC ID: 8344160

PubMed ID: 34362454

SO-VID: 49e9bd44-9bfa-4de6-ab35-afb84f107c27

License:

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

History

Date received : 28 April 2021

Date accepted : 11 July 2021

Funding

Funded by: FundRef http://dx.doi.org/10.13039/501100001809, National Natural Science Foundation of China;

Award ID: 81871576

Award ID: 81701901

Award ID: 81801946

Funded by: Shanghai Municipal Key Clinical Specialty

Award ID: shslczdzk00901

Custom metadata

ScienceOpen disciplines: Molecular medicine

Keywords: dermatofibrosarcoma protuberans (dfsp),adipose tissue-derived stem cells (adscs),skin therapy,regenerative medicine

Data availability:

ScienceOpen disciplines: Molecular medicine

Keywords: dermatofibrosarcoma protuberans (dfsp), adipose tissue-derived stem cells (adscs), skin therapy, regenerative medicine

Impact of human adipose tissue-derived stem cells on dermatofibrosarcoma protuberans cells in an indirect co-culture: an in vitro study

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Abstract

Background

Methods

Results

Conclusions

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Most cited references 72

Fibroblasts in cancer.

In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro.

Angiogenesis: an organizing principle for drug discovery?

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