Methane-cycling microbial communities from Amazon floodplains and upland forests respond differently to simulated climate change scenarios

Abstract

Seasonal floodplains in the Amazon basin are important sources of methane (CH ₄), while upland forests are known for their sink capacity. Climate change effects, including shifts in rainfall patterns and rising temperatures, may alter the functionality of soil microbial communities, leading to uncertain changes in CH ₄ cycling dynamics. To investigate the microbial feedback under climate change scenarios, we performed a microcosm experiment using soils from two floodplains (i.e., Amazonas and Tapajós rivers) and one upland forest. We employed a two-factorial experimental design comprising flooding (with non-flooded control) and temperature (at 27 °C and 30 °C, representing a 3 °C increase) as variables. We assessed prokaryotic community dynamics over 30 days using 16S rRNA gene sequencing and qPCR. These data were integrated with chemical properties, CH ₄ fluxes, and isotopic values and signatures. In the floodplains, temperature changes did not significantly affect the overall microbial composition and CH ₄ fluxes. CH ₄ emissions and uptake in response to flooding and non-flooding conditions, respectively, were observed in the floodplain soils. By contrast, in the upland forest, the higher temperature caused a sink-to-source shift under flooding conditions and reduced CH ₄ sink capability under dry conditions. The upland soil microbial communities also changed in response to increased temperature, with a higher percentage of specialist microbes observed. Floodplains showed higher total and relative abundances of methanogenic and methanotrophic microbes compared to forest soils. Isotopic data from some flooded samples from the Amazonas river floodplain indicated CH ₄ oxidation metabolism. This floodplain also showed a high relative abundance of aerobic and anaerobic CH ₄ oxidizing Bacteria and Archaea. Taken together, our data indicate that CH ₄ cycle dynamics and microbial communities in Amazonian floodplain and upland forest soils may respond differently to climate change effects. We also highlight the potential role of CH ₄ oxidation pathways in mitigating CH ₄ emissions in Amazonian floodplains.

Supplementary Information

The online version contains supplementary material available at 10.1186/s40793-024-00596-z.