Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening

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Abstract

This protocol from Feng Zhang's lab enables genome-scale knockout and transcriptional activation screening using the CRISPR-Cas9 system, as sgRNA libraries are constructed and packaged into lentiviral vectors for delivery into cells for screening.

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Journal

Title: Nature Protocols

Abbreviated Title: Nat Protoc

Publisher: Springer Nature

ISSN (Print): 1754-2189

ISSN (Electronic): 1750-2799

Publication date Created: March 23 2017

Publication date (Print): March 23 2017

Volume: 12

Issue: 4

Pages: 828-863

Article

DOI: 10.1038/nprot.2017.016

PMC ID: 5526071

PubMed ID: 28333914

SO-VID: 45a36bd7-152f-4c91-b9a1-db6185d8185d

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