In spite of the benefits associated with exclusive breastfeeding practice (EBP), the practice remains low in Ghana. This study investigates prevalence and factors associated with EBP among lactating mothers of infants aged 6-24 months in Metropolitan Kumasi. Cross-sectional hospital-based data were collected at 5 health facilities from 160 randomly sampled lactating mothers. Multivariate logistic regressions were performed to determine factors associated with EBP.