Murine CD146 is widely expressed on endothelial cells and is recognized by the monoclonal antibody ME-9F1

There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

Abstract

The endothelium plays an important role in the exchange of molecules, but also of immune cells between blood and the underlying tissue. The endothelial molecule S-Endo 1 antigen (CD146) is preferentially located at endothelial junctions and has been claimed to support endothelial integrity. In this study we show that the monoclonal antibody ME-9F1 recognizes the extracellular portion of murine CD146. Making use of ME-9F1 we found CD146 highly expressed and widely spread on endothelial cells in the analyzed murine tissues. In contrast to humans that express CD146 also on T cells or follicular dendritic cells, murine CD146 albeit at low levels was only found on a subset of NK1.1 ⁺ cells. The antibody against murine CD146 is useful for immunomagnetic sorting of primary endothelial cells not only from the liver but from various other organs. In vitro, no evidence was seen that the formation and integrity of endothelial monolayers or the transendothelial migration of T cells was affected by antibody binding to CD146 or by crosslinking of the antigen. This makes the antibody ME-9F1 an excellent tool especially for the ex vivo isolation of murine endothelial cells intended to be used in functional studies.

Related collections

Most cited references 44

Record: found
Abstract: found
Article: not found

Hepatic T cells and liver tolerance.

I. Nicholas Crispe (2002)

The T-cell biology of the liver is unlike that of any other organ. The local lymphocyte population is enriched in natural killer (NK) and NKT cells, which might have crucial roles in the recruitment of circulating T cells. A large macrophage population and the efficient trafficking of dendritic cells from sinusoidal blood to lymph promote antigen trapping and T-cell priming, but the local presentation of antigen causes T-cell inactivation, tolerance and apoptosis. These local mechanisms might result from the need to maintain immunological silence to harmless antigenic material in food. The overall bias of intrahepatic T-cell responses towards tolerance might account for the survival of liver allografts and for the persistence of some liver pathogens.

0 comments Cited 151 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: not found

CD105 is important for angiogenesis: evidence and potential applications.

Chenggang Li, Shant Kumar, Martin J. Garland … (2003)

Angiogenesis is the propelling force for tumor growth and metastasis, and antiangiogenic therapy represents one of the most promising modalities for cancer treatment. CD105 (endoglin) is a proliferation-associated and hypoxia-inducible protein abundantly expressed in angiogenic endothelial cells (EC). It is a receptor for transforming growth factor (TGF) -beta1 and -beta3 and modulates TGF-beta signaling by interacting with TGF-beta receptors I and/or II. Immunohistochemistry studies have revealed that CD105 is strongly expressed in blood vessels of tumor tissues. Intratumoral microvessel density (MVD) determined using antibodies to CD105 has been found to be an independent prognostic indicator, wherein increased MVD correlates with shorter survival. CD105 is able to be shed into the circulation, with elevated levels detected in patients with various types of cancer and positively correlated with tumor metastasis. Tangible evidence of its proangiogenic role comes from knockout studies in which CD105 null mice die in utero as a result of impaired angiogenesis in the yolk sac and heart defects. The potential usefulness of CD105 for tumor imaging has been evaluated in tumor-bearing mice and dogs that have shown the rapid accumulation of radiolabeled anti-CD105 monoclonal antibody in the tumors with a high tumor-to-background ratio. The anti-CD105 antibody conjugated with immunotoxins and immunoradioisotopes efficiently suppressed/abrogated tumor growth in murine models bearing breast and colon carcinoma without any significant systemic side effects. Immunoscintigraphy in patients with renal cell carcinomas has shown specific localization of 99Tcm-labeled CD105 mab in tumor endothelial cells. Thus, CD105 is a promising vascular target that can be used for tumor imaging, prognosis, and bears therapeutic potential in patients with solid tumors and other angiogenic diseases.

0 comments Cited 116 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: not found

The molecular organization of endothelial cell to cell junctions: differential association of plakoglobin, beta-catenin, and alpha- catenin with vascular endothelial cadherin (VE-cadherin)

M Corada, MG Lampugnani, L Caveda … (1995)

In this paper we report that the assembly of interendothelial junctions containing the cell type-specific vascular endothelial cadherin (VE- cadherin or cadherin-5) is a dynamic process which is affected by the functional state of the cells. Immunofluorescence double labeling of endothelial cells (EC) cultures indicated that VE-cadherin, alpha- catenin, and beta-catenin colocalized in areas of cell to cell contact both in sparse and confluent EC monolayers. In contrast, plakoglobin became associated with cell-cell junctions only in tightly confluent cells concomitantly with an increase in its protein and mRNA levels. Furthermore, the amount of plakoglobin coimmunoprecipitated with VE- cadherin, increased in closely packed monolayers. Artificial wounding of confluent EC monolayers resulted in a major reorganization of VE- cadherin, alpha-catenin, beta-catenin, and plakoglobin. All these proteins decreased in intensity at the boundaries of EC migrating into the lesion. In contrast, EC located immediately behind the migrating front retained junctional VE-cadherin, alpha-catenin, and beta-catenin while plakoglobin was absent from these sites. In line with this observation, the amount of plakoglobin coimmunoprecipitated with VE- cadherin decreased in migrating EC. These data suggest that VE- cadherin, alpha-catenin, and beta-catenin are already associated with each other at early stages of intercellular adhesion and become readily organized at nascant cell contacts. Plakoglobin, on the other hand, associates with junctions only when cells approach confluence. When cells migrate, this order is reversed, namely, plakoglobin dissociates first and, then, VE-cadherin, alpha-catenin, and beta-catenin disassemble from the junctions. The late association of plakoglobin with junctions suggests that while VE-cadherin/alpha-catenin/beta- catenin complex can function as an early recognition mechanism between EC, the formation of mature, cytoskeleton-bound junctions requires plakoglobin synthesis and organization.

0 comments Cited 92 times – based on 0 reviews      Review now

Bookmark

All references

Author and article information

Contributors

: +49-30-84454026 , +49-30-84454017 , katja.klugewitz@charite.de

Journal

Journal ID (nlm-ta): Histochem Cell Biol

Title: Histochemistry and Cell Biology

Publisher: Springer-Verlag (Berlin/Heidelberg )

ISSN (Print): 0948-6143

ISSN (Electronic): 1432-119X

Publication date (Electronic): 24 January 2008

Publication date (Print): April 2008

Volume: 129

Issue: 4

Pages: 441-451

Affiliations

[1 ]Gastroenterologie, Charité Campus, Benjamin Franklin, Hindenburgdamm 30, 12203 Berlin, Germany

[2 ]Institut für Pathologie, Charité Campus Benjamin Franklin, Hindenburgdamm 30, 12203 Berlin, Germany

[3 ]Rheumatologie, Charité Campus Mitte, Schumannstr. 21/22, 10117 Berlin, Germany

[4 ]Max-Planck-Institut für Infektionsbiologie, Schumannstr. 21/22, 10117 Berlin, Germany

[5 ]Institut für Immunologie, Ludwig-Maximilians-Universität, Goethestr. 29-31, 80336 Munich, Germany

[6 ]Institut für Molekulare Medizin und Experimentelle Immunologie, Rheinische Friedrich-Wilhelms-Universität, Sigmund Freud Str. 25, 53105 Bonn, Germany

Article

Publisher ID: 379

DOI: 10.1007/s00418-008-0379-x

PMC ID: 2756363

PubMed ID: 18214516

SO-VID: 42d46fc6-7e5f-418c-93e7-b04a58ef0d18

History

Date accepted : 7 January 2008

Custom metadata

ScienceOpen disciplines: Cell biology

Keywords: endothelial cells,antibody me-9f1,tissue distribution of murine cd146,nk cells

Data availability:

ScienceOpen disciplines: Cell biology

Keywords: endothelial cells, antibody me-9f1, tissue distribution of murine cd146, nk cells

Murine CD146 is widely expressed on endothelial cells and is recognized by the monoclonal antibody ME-9F1

Read this article at

Abstract

Related collections

Stress signalling in stem cells

Most cited references 44

Hepatic T cells and liver tolerance.

CD105 is important for angiogenesis: evidence and potential applications.

The molecular organization of endothelial cell to cell junctions: differential association of plakoglobin, beta-catenin, and alpha- catenin with vascular endothelial cadherin (VE-cadherin)

Author and article information

Contributors

Journal

Affiliations

Article

History

Categories

Custom metadata

Comments

Comment on this article

Similar content 610

Cited by 28

Most referenced authors 453