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      Who are you, subdistal appendages of centriole?

      review-article
      1 , 2 , 3
      Open Biology
      The Royal Society
      centriole, distal appendages, subdistal appendages

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          Abstract

          This review summarizes data that assign morphological, biochemical and functional characteristics of two types of structures that are associated with centrioles: distal appendages and subdistal appendages. The description of centriole subdistal appendages is often a matter of confusion, both due to the numerous names used to describe these structures and because of their variability among species and cell types. Thus, we have summarized our current knowledge in this review. We conclude that distal appendages and subdistal appendages are fundamentally different in composition and function in the cell. While in centrioles there are always nine distal appendages, the number of subdistal appendages can vary depending on the type of cells and their functional state.

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          Most cited references61

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          Cep164, a novel centriole appendage protein required for primary cilium formation

          Primary cilia (PC) function as microtubule-based sensory antennae projecting from the surface of many eukaryotic cells. They play important roles in mechano- and chemosensory perception and their dysfunction is implicated in developmental disorders and severe diseases. The basal body that functions in PC assembly is derived from the mature centriole, a component of the centrosome. Through a small interfering RNA screen we found several centrosomal proteins (Ceps) to be involved in PC formation. One newly identified protein, Cep164, was indispensable for PC formation and hence characterized in detail. By immunogold electron microscopy, Cep164 could be localized to the distal appendages of mature centrioles. In contrast to ninein and Cep170, two components of subdistal appendages, Cep164 persisted at centrioles throughout mitosis. Moreover, the localizations of Cep164 and ninein/Cep170 were mutually independent during interphase. These data implicate distal appendages in PC formation and identify Cep164 as an excellent marker for these structures.
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            Staining of Tissue Sections for Electron Microscopy with Heavy Metals

            Heavy metals may be incorporated from solution into tissue sections for electron microscopy. The resulting increase in density of the tissue provides greatly enhanced contrast with minimal distortion. Relative densities of various structures are found to depend on the heavy metal ions present and on the conditions of staining. Certain hitherto unobserved details are revealed and some sort of specificity exists, although the factors involved are not yet understood.
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              Centriole distal appendages promote membrane docking, leading to cilia initiation.

              The distal appendages (DAPs) of centrioles have been proposed to anchor cilia to the plasma membrane, but their molecular composition, assembly, and exact function in ciliogenesis remain poorly understood. Using quantitative centrosome proteomics and superresolution microscopy, we identified five DAP components, including one previously described (CEP164), one partially characterized (CEP89 [ccdc123]), and three novel (CEP83 [ccdc41], SCLT1, and FBF1) DAP proteins. Analyses of DAP assembly revealed a hierarchy. CEP83 recruits both SCLT1 and CEP89 to centrioles. Subsequent recruitment of FBF1 and CEP164 is independent of CEP89 but mediated by SCLT1. All five DAP components are essential for ciliogenesis; loss of CEP83 specifically blocks centriole-to-membrane docking. Undocked centrioles fail to recruit TTBK2 or release CP110, the two earliest modifications found on centrioles prior to cilia assembly, revealing centriole-to-membrane docking as a temporal and spatial cue promoting cilia initiation.
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                Author and article information

                Journal
                Open Biol
                Open Biol
                RSOB
                royopenbio
                Open Biology
                The Royal Society
                2046-2441
                July 2018
                25 July 2018
                25 July 2018
                : 8
                : 7
                : 180062
                Affiliations
                [1 ]Faculté de Médecine, Université de Tours , 10 Boulevard Tonnellé, 37032 Tours, France
                [2 ]Faculty of Bioengineering and Bioinformatics, Moscow State University , Leninskye gory 73, 119234 Moscow, Russia
                [3 ]Belozersky Institute of Physico-Chemical Biology, Moscow State University , Leninskye gory 1-40, 119992 Moscow, Russia
                Author notes
                Author information
                http://orcid.org/0000-0002-9336-5484
                Article
                rsob180062
                10.1098/rsob.180062
                6070718
                30045886
                3feb3ace-26fc-470b-97c5-192a2cc256fc
                © 2018 The Authors.

                Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

                History
                : 11 April 2018
                : 29 June 2018
                Categories
                33
                201
                Review
                Review Article
                Custom metadata
                July 2018

                Life sciences
                centriole,distal appendages,subdistal appendages
                Life sciences
                centriole, distal appendages, subdistal appendages

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