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      Activation of glucokinase gene expression by hepatic nuclear factor 4alpha in primary hepatocytes.

      Biochemical Journal
      Animals, Base Sequence, Binding Sites, genetics, Cells, Cultured, DNA-Binding Proteins, Gene Expression Regulation, Enzymologic, Glucokinase, metabolism, Hepatocyte Nuclear Factor 4, Hepatocytes, Oxygen, Phosphoproteins, Promoter Regions, Genetic, RNA, Messenger, Rats, Recombinant Proteins, Trans-Activators, Transcription Factors, Transfection

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          Abstract

          Glucokinase (GK) is a key enzyme for glucose utilization in liver and shows a higher expression in the perivenous zone. In primary rat hepatocytes, the GK gene expression was activated by HNF (hepatic nuclear factor)-4alpha via the sequence -52/-39 of the GK promoter. Venous pO2 enhanced HNF-4 levels and HNF-4 binding to the GK-HNF-4 element. Thus, HNF-4alpha could play the role of a regulator for zonated GK expression.

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