Biomechanical Weakening of Paper and Plant Cell Walls by Bacterial Expansins.

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Abstract

Expansins are proteins that loosen plant cell walls but lack enzymatic activity. Here we describe two protocols tailored to measure the biomechanical activity of bacterial expansin. The first assay relies on the weakening of filter paper by expansin. The second assay is based on induction of creep (long-term, irreversible extension) of plant cell wall samples.

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Author and article information

Journal

Journal ID (iso-abbrev): Methods Mol Biol

Title: Methods in molecular biology (Clifton, N.J.)

Publisher: Springer Science and Business Media LLC

ISSN (Electronic): 1940-6029

ISSN (Print): 1064-3745

Publication date (Electronic): 2023

Volume: 2657

Affiliations

[1 ] Department of Biology, Pennsylvania State University, University Park, PA, USA. dcosgrove@psu.edu.

[2 ] Department of Biology, Pennsylvania State University, University Park, PA, USA.

Article

DOI: 10.1007/978-1-0716-3151-5_5

PubMed ID: 37149523

SO-VID: 3f0238fc-7913-4362-9ae2-f02035cbf747

History

Keywords: Filter paper,Plant cell wall,Wall loosening proteins,Cellulose,Creep,Expansin

Data availability:

Keywords: Filter paper, Plant cell wall, Wall loosening proteins, Cellulose, Creep, Expansin

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